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为了测定和比较不同烟叶及国内外代表性卷烟中的吡嗪、吡啶类香味成分含量的差异性,建立了烟草中吡嗪和吡啶类物质的同时蒸馏萃取-气相色谱-质谱联用(SDE-GC-M S)测定法,优化了同时蒸馏萃取液的净化方法以及仪器测定条件。化合物的线性系数范围为0.9957~0.9999,RSD范围为5.3%~8.6%,回收率范围为73.0%~101.4%,检出限范围为0.0015~0.0029μg/g,可以满足烟草中吡啶、吡嗪类物质的定量测定。研究发现,产地对烟叶中吡嗪、吡啶类化合物含量影响较大;烟叶不同部位中吡嗪、吡啶类化合物差异性显著;不同品种烟叶中吡嗪、吡啶类化合物含量差异性较大,晒黄烟主要是吡啶类化合物含量较高,白肋烟中吡嗪类化合物含量相对较高;混合型卷烟烟丝中吡嗪、吡啶类香味成分含量明显高于烤烟型卷烟。
In order to determine and compare the differences of the contents of pyrazines and pyridines in different tobacco leaves and domestic and foreign representative cigarettes, the simultaneous distillation extraction-gas chromatography-mass spectrometry (SDE- GC-M S) method to optimize the method of simultaneous purification of distillate extracts and instrument determination conditions. The linear range of the compounds ranged from 0.9957 to 0.9999, RSD ranged from 5.3% to 8.6%, and the recoveries ranged from 73.0% to 101.4%. The detection limits ranged from 0.0015 to 0.0029 μg / g, which could meet the requirements of pyridine, pyrazine Quantitative determination of substances. The study found that the origin of the tobacco leaf pyrazines, pyridine compounds greater impact; different parts of the tobacco pyrazines, pyridine compounds significantly different; different varieties of tobacco pyrazines, pyridine compounds greater differences in the amount of sun yellow smoke The content of pyrazines in burley tobacco was higher than that in burley tobacco. The contents of pyrazines and pyridine in the mixed-type cigarette were significantly higher than that in flue-cured tobacco.