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目的:寻找作为SD大鼠胚胎-胎仔发育毒性试验(Ⅱ段生殖毒性)较理想的阳性对照药物。方法:在大鼠胚胎-胎仔发育毒性试验中,分别在妊娠第10日给予不同剂量敌枯双和环磷酰胺,于妊娠第20日解剖观察胎仔畸形,从致畸种类、致畸率等指标分析比较致畸作用。结果:敌枯双11.0mg/kg组和13.8mg/kg组外观畸形率分别为84.62%和86.84%(P<0.01),而且种类较多,如:脊柱裂、心脏外露、尾畸形、肛门闭锁、骨骼畸形等;环磷酰胺组发生的畸形较少,以骨骼发育迟缓为主。结论:敌枯双对大鼠的致畸率高、致畸种类多,是较好的阳性对照药物。
OBJECTIVE: To find out the ideal positive control drug for the embryonic development of fetal rats - fetal development toxicity test (reproductive toxicity of stage Ⅱ). Methods: In the embryo-fetal developmental toxicity test, different dosages of dizocilpine and cyclophosphamide were given on the 10th day of gestation respectively. Anatomy of the fetus was observed on the 20th day of gestation, including teratogenicity and teratogenicity Analysis of teratogenic effects. Results: The appearance deformity rates in the group of Zuiquang 11.0mg / kg and 13.8mg / kg were 84.62% and 86.84%, respectively (P <0.01), and there were more species such as spina bifida, cardiac exposure, caudate deformity, anal atresia , Skeletal deformities, etc .; cyclophosphamide group deformity less to skeletal development-based. CONCLUSION: Zuokulian has high teratogenicity and teratogenicity in rats, which is a good positive control drug.