论文部分内容阅读
目的探讨多探针荧光原位杂交(FISH)技术联合G显带核型分析(CCG)在喀什地区维吾尔族成人急性髓系白血病(AML)诊断中的应用价值。方法采用AML多探针FISH系统[包括PML/RARα、AML1/ETO、CBFβ/MYH11融合基因,MLL基因重排,Del(20q),-7/Del(7q),Del(5q)及P53基因8种探针]对30例该地区新诊断维吾尔族成人AML进行检测,同期进行常规CCG。结果 30例AML中有20例多探针FISH检测出细胞遗传学异常,异常检出率为66.7%。而CCG的异常检出率为36.7%,相对应的遗传学异常仅检出9例,另检出2例多探针FISH不能检出的异常。多探针FISH异常检出率明显高于CCG(P<0.05),且两者结合可将检出率提高至73.3%。结论多探针FISH对于细胞遗传学异常的检出率较CCG高,两者联合检测可以提高AML遗传学异常的检出率,为该地区维吾尔族成人AML的精准诊断提供更多客观可靠的依据。
Objective To investigate the value of multi-probe fluorescence in situ hybridization (FISH) combined with G-banding karyotype analysis (CCG) in the diagnosis of Uygur adult acute myeloid leukemia (AML) in Kashgar region. Methods AML multi-probe FISH system [including PML / RARα, AML1 / ETO, CBFβ / MYH11 fusion gene, MLL gene rearrangement, Del (20q), - 7 / Del Probe] in 30 cases of newly diagnosed Uighur adult AML in this area, the same period for conventional CCG. Results Twenty cases of AML were detected by multi-probe FISH. The abnormality rate was 66.7%. The detection rate of CCG was 36.7%. Only nine cases were detected in the corresponding genetic abnormalities, and the other two cases were not detected by FISH. The detection rate of multi-probe FISH was significantly higher than that of CCG (P <0.05), and the combination of both could increase the detection rate to 73.3%. Conclusion The detection rate of multi-probe FISH for cytogenetic abnormalities is higher than that of CCG. The combined detection of these two methods can increase the detection rate of AML genetic abnormalities and provide more objective and reliable evidences for the accurate diagnosis of Uighur adult AML .