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目的：探讨冷束缚应激大鼠血浆及胃粘膜中β－内啡肽含量的变化及作用。方法：制作大鼠冷束缚应激模型，以放射免疫法测定应激０ｍｉｎ（对照组）、１５ ｍｉｎ、２ｈ及８ｈ时大鼠血浆及胃粘膜中β－内啡肽的含量，同时测定胃液ｐＨ和胃粘膜溃疡指数（ＵＩ）。另设两组预处理组，分别于应激前１５ ｍｉｎ经颈静脉注射纳洛酮（１．６ ｍｇ／ｋｇ）或等容量生理盐水（０．８ｍｇ／ｋｇ），测定其应激２ｈ时的胃液ｐＨ及胃粘膜ＵＩ。结果：整个应激过程中，大鼠血浆及胃粘膜中β－内啡肽含量无显著变化（Ｐ＞０．０５）；应激各组大鼠的胃粘膜ＵＩ均显著高于对照组（Ｐ＜０.０１）；应激１５ ｍｉｎ组大鼠的胃液ｐＨ显著高于对照组（Ｐ＜０．０１），临终前（应激８ｈ组）大鼠的胃液ｐＨ亦显著高于对照组（Ｐ＜０．０５）；纳洛酮组大鼠的胃液ｐＨ及胃粘膜ＵＩ较生理盐水对照组无显著差异（Ｐ＞０．０５）。结论：应激过程中大鼠血浆及胃粘膜中β－内啡肽含量稳定，阻断β－内啡肽受体不影响胃液ｐＨ及胃粘膜ＵＩ；β－内啡肽对应激所致的胃粘膜损伤可能不起重要作用。
Objective: To investigate the changes and effects of β-endorphin in plasma and gastric mucosa of cold-restraint stress-induced rats. Methods: Cold stress model was established in rats. Radioimmunoassay was used to determine the content of β-endorphin in plasma and gastric mucosa of rats at 0 min (control), 15 min, 2 h and 8 h, And gastric ulcer index (UI). In addition, two groups of preconditioning group were injected with naloxone (1.6 mg / kg) or normal saline (0.8 mg / kg) intravenously 15 min before the stress, respectively. Gastric pH and gastric mucosal UI. Results: The content of β-endorphin in plasma and gastric mucosa did not change significantly during the whole stress (P> 0.05). The gastric mucosal UI of rats in each stress group was significantly higher than that of the control group (P (P <0.01). The gastric pH of rats in 15 min stress group was significantly higher than that of control group (P <0.01) <0.05). The gastric pH and mucosal UI of naloxone group were not significantly different from those of saline control group (P> 0.05). CONCLUSIONS: The content of β-endorphin in plasma and gastric mucosa of rats during the stress is stable, the β-endorphin receptor blockade does not affect the gastric pH and gastric mucosal UI; β-endorphin exerts an effect on the stress-induced gastric Mucosal injury may not play an important role.