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目的:观察肺癌组织热休克蛋白70(HSP70)提取物对A549细胞增殖的影响。方法:从肺癌组织中提取、纯化HSP70多肽复合物,分别以0.1、0.5、1.0、2.5及5.0mg/L作用于A549细胞,采用MTT法检测作用24、48及72h后细胞的增殖。结果:HSP70多肽复合物可促进A549细胞增殖,其作用随浓度和时间的增加呈增强的趋势(F浓度=171.826,F时间=35.933,P<0.001)。结论:HSP70多肽复合物可以促进A549细胞的增殖。
Objective: To observe the effect of heat shock protein 70 (HSP70) extract on the proliferation of A549 cells in lung cancer. Methods: HSP70 peptide complex was extracted and purified from lung cancer tissues. A549 cells were treated with 0.1, 0.5, 1.0, 2.5 and 5.0 mg / L respectively. The proliferation of the cells was detected by MTT assay after 24, 48 and 72 hours. Results: HSP70 peptide complex could promote the proliferation of A549 cells. The effect of HSP70 increased with the increase of concentration and time (F concentration = 171.826, F time = 35.933, P <0.001). Conclusion: HSP70 peptide complex can promote the proliferation of A549 cells.