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用4,7-二苯基-1,10-菲啰啉(BPT)为试剂萃取分光光度法测定水、血清、高纯氧化锌中微量铁已有报导,但手续繁杂。用非离子表面活性剂Triton X-100胶束增溶BPT-Fe体系分光光度法未见报导。本文用Triton X-100增溶BPT-Fe体系,发现在pH为1.9-4.7乙酸介质中,Fe-BPT-Triton X-100可形成稳定的三元胶束络合物。在532.2nm处有最大吸收,摩尔吸光系数ε_(532.2)为2.2×10~4。除Cu~+离子外,其他常见离子均不干扰。用于测定天然水及海洋生物中微量铁,结果良好。
Spectrophotometric determination of trace iron in serum, serum and high purity zinc oxide using 4,7-diphenyl-1,10-phenanthroline (BPT) as a reagent has been reported, but the procedures are complicated. The non-ionic surfactant Triton X-100 micellar solubilization BPT-Fe system spectrophotometry has not been reported. In this paper, Triton X-100 was used to solubilize BPT-Fe system. It was found that Fe-BPT-Triton X-100 can form a stable ternary micelle complex in a pH range of 1.9-4.7 acetic acid. The maximum absorption at 532.2nm, the molar extinction coefficient ε_ (532.2) of 2.2 × 10 ~ 4. In addition to Cu ~ + ions, other common ions do not interfere. For the determination of trace amounts of trace iron in natural water and marine organisms, the result is good.