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【答】鲎试验,即鲎变形细胞溶解物试验(英文缩写LLT),是近年国内开展的一种新的检测微量内毒素的方法.具有简单、诊断快速、敏感性高、不受抗生素干扰等优点.1956年国外有人首先发现给美洲鲎注射革兰氏阴性(G~-)细菌可引起致命性血管内凝血,1970年用于检测人体的内毒素血症.国内于1978年开展血浆、体液(胸腹水、脑脊液、胆汁、尿液)以及药品,生物制品等内毒素检测.其原理系细菌内毒素作用于鲎的循环血细胞(即变形细胞),随着细胞溶解物的出现,内毒素可激活凝固酶原变成凝固酶,通过凝固酶的作用,使凝固蛋白原变成凝固蛋白而形成凝胶.鲎试剂的灵敏度很高,每毫升内含0.3~0.003纳克(ng)的内毒素即能被检出.内毒素是G~-细菌细胞壁的脂多糖成
【Answer】 答 test, that is, 鲎 deformation cell lysate test (abbreviation LLT), is a new method of detection of trace endotoxin in China in recent years with a simple, rapid diagnosis, high sensitivity, free from antibiotics and other interference Advantages In 1956, foreign people first discovered that injecting Gram-negative bacteria (G ~) to American pomfret can cause fatal intravascular coagulation and used to detect endotoxemia in the human body in 1970. In 1978, the plasma, body fluid (Pleural effusion, cerebrospinal fluid, bile, urine), as well as drugs, biological products such as endotoxin detection. The principle of bacterial endotoxin acting on the circulation of blood cells (ie, deformed cells), with the emergence of cell lysate, endotoxin can Activation of coagulase into coagulase, coagulant effect, coagulogen turns into coagulation protein and form a gel. 鲎 reagent sensitivity is high, per milliliter contains 0.3 ~ 0.003 ng (ng) of endotoxin That can be detected endotoxin is G ~ - bacterial cell wall lipopolysaccharide into