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利用株型差异显著的特大粒粳稻品系TD70和籼稻小粒品种Kasalath为亲本配制组合,以单粒传方法构建含240个株系的重组自交系(RIL)群体选用838对SSR引物进行亲本多态性筛选,共检测到302对具有多态性的引物,频率为36.04%从中选择带型清晰且在基因组中均匀分布的141个SSR标记对RIL群体进行基因型分析,结果表明:群体中父母本基因频率分别为53%和47%,群体结构平衡性好构建的水稻分子连锁图谱共包含141个标记座位,总图距约1832.47cM,标记间平均图距为12.7cM,标记间图距范围为0.43～36.11cM,符合QTL作图的基本要求除第1、第8染色体个别标记位置外,其他染色体上标记顺序和位置与已公布的日本晴遗传图谱序列基本一致以该群体为材料,对分蘖角度进行了QTL检测,共检测到控制分蘖角度的3个QTL位点,分别是qTA8、qTA9和qTA11,贡献率分别为4.10%、26.08%和4.35%,其中qTA9包含控制水稻分蘖角度基因TAC1。该图谱的构建为研究籼粳交后代各种性状的遗传规律及QTL定位打下了基础。
Using a combination of TD70, an extra-large japonica rice line with a significant difference in plant type, and Kasalath, an indica small-grain variety, the recombinant inbred line (RIL) population with 240 lines was constructed by single grain propagation. 838 pairs of SSR primers were used for the parental polymorphism A total of 302 pairs of polymorphic primers were detected at a frequency of 36.04%. A total of 141 SSR markers with clear bands and even distribution in the genome were selected to genotype RIL population. The results showed that: The gene frequency was 53% and 47%, respectively. The rice molecular linkage map constructed by a well-balanced population structure contained a total of 141 marker loci with a total map distance of about 1832.47cM. The average distance between markers was 12.7cM. 0.43 ~ 36.11cM, consistent with the basic requirements of QTL mapping Except for the first and eighth chromosomal marker locations, other chromosomes on the order and location of markers and the published sequence of Nipponbare map is basically the same as the group of materials, the tiller angle QTLs were detected and three QTLs controlling tillering angles were detected, which were qTA8, qTA9 and qTA11, with the contribution rates of 4.10%, 26.08% and 4.35%, respectively. Among them, qTA9 included the control of rice tiller The degree of gene TAC1. The construction of this map lays the foundation for studying the genetic rules and QTL mapping of various traits in offspring of indica and japonica hybrids.