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目的:观察比较活血化瘀方、清热祛湿方和养阴柔肝方3种不同治法中药复方对H22荷瘤小鼠瘤细胞增殖的影响。方法:采用皮下接种肝癌H22细胞的方法建立荷瘤小鼠模型,分别用上述3种中药复方水煎液灌胃处理11 d,观察各组荷瘤小鼠的肿瘤生长情况,用免疫组织化学方法检测肿瘤组织增殖细胞核抗原(PCNA)及G1期细胞周期蛋白(CyclinD1)的表达情况。结果:3种复方作用后,活血化瘀方对瘤细胞PCNA表达的抑制作用最为显著;用药组小鼠瘤细胞CyclinD1阳性表达细胞百分数与单纯造模组比较,差异具有统计学意义(P<0.01),3个给药组之间两两比较差异亦具有统计学意义(P<0.01)。结论:3种治法中药复方在抑制瘤细胞增殖方面以活血化瘀方作用最为显著;3者均可以明显抑制肝癌细胞CyclinD1表达,推断3种方法能够抑制肝癌细胞增殖活性的分子机制,可能是通过降低肝癌细胞内CyclinD1的阳性表达,阻断CyclinD1-CDK4-Rb通路,降低细胞周期转换速度实现的。
OBJECTIVE: To observe the effects of three recipes of Huoxue Huayu Fang, Qingre Qushi Decoction and Yangyin Rougan Recipe on the proliferation of tumor cells in H22 tumor-bearing mice. METHODS: A tumor-bearing mouse model was established by subcutaneously inoculating hepatoma H22 cells. The three kinds of traditional Chinese medicine compound decoction were intragastrically treated for 11 days. The tumor growth of tumor-bearing mice in each group was observed and immunohistochemistry was used. The expression of proliferating cell nuclear antigen (PCNA) and G1 phase cyclin (CyclinD1) was detected in tumor tissue. Results: After the three kinds of compound prescriptions, the effect of Huoxue Huayu Decoction on the expression of PCNA in tumor cells was the most significant; the percentage of CyclinD1 positive cells in mouse tumor cells of the treatment group was compared with that of the model group alone, the difference was statistically significant (P<0.01). ), The difference between the two groups was also statistically significant (P<0.01). Conclusion: The three kinds of traditional Chinese medicine compound recipes have the most significant effect in inhibiting the proliferation of tumor cells by promoting blood circulation and removing blood stasis. All three can significantly inhibit the expression of CyclinD1 in hepatocellular carcinoma cells, and the molecular mechanism of inhibition of the proliferation activity of hepatoma cells by three methods may be inferred. By reducing the positive expression of CyclinD1 in hepatoma cells, blocking the CyclinD1-CDK4-Rb pathway and reducing the cell cycle turnover rate.