论文部分内容阅读
目的：探讨P16蛋白与肝细胞性肝癌生物学行为的关系。方法：应用P16多克隆抗体对60例肝癌用S－P法进行免疫组织化学标记。结果：60例肝细胞性肝癌中有39例P16蛋白阳性（65％）。根据肿瘤大小分为3组，即肿瘤直径＜3cm，直径3～5cm及直径＞5cm。其P16表达阳率率分虽为75％、62.5％、60％，三者之间无显著性意义（P＞0．05）。根据组织学类型分梁状型、假腺管型、实团型与纤维硬化型，其P16表达阳性率分别为95.2％、81．8％、36.4％和33.3％。前两组与后两组之间有统计学差异（P＜0.01）。根据组织的分化程度，高分化（包括Ⅰ～Ⅱ级）P16阳性率明显高于低分化（包括Ⅲ～Ⅳ级）。结论：提示P16蛋白的表达与肝细胞性肝癌的分化程度有关，与肿瘤大小有关。
Objective: To investigate the relationship between P16 protein and the biological behavior of hepatocellular carcinoma. Methods: 60 cases of liver cancer were immunohistochemically labeled with S-P method using P16 polyclonal antibody. Results: Of the 60 cases of hepatocellular carcinoma, 39 were positive for P16 protein (65%). According to the tumor size was divided into 3 groups, namely tumor diameter <3cm, diameter 3 ~ 5cm and diameter> 5cm. The rate of positive rate of P16 expression was 75%, 62.5%, and 60%, but there was no significant difference among them (P>0.05). According to the histological types, the positive rates of P16 expression were 95.2%, 81.8%, 36.4%, and 33.3% in beams, false glands, plexus, and fibrosis. There was a statistical difference between the first two groups and the latter two groups (P<0.01). According to the degree of tissue differentiation, the positive rate of high-differentiation (including grade I-II) P16 was significantly higher than that of poor differentiation (including grades III-IV). Conclusion: It is suggested that the expression of P16 protein is related to the degree of differentiation of hepatocellular carcinoma and is related to the size of the tumor.