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通过对浙农12等七个无性系茶树品种扦插发根过程的显微切片观察和主要生化指标的测定,结果表明:茶树红棕色枝和绿色枝的不定根原基分别起源于木栓形成层和中柱鞘内侧的韧皮薄壁细胞。根据插穗基部解剖学和形态学特征的变化,茶树插穗不定根的形成过程可分为5个时期,即:根原基未分化期、根原基分化期、根原体分化前期、根原体分化后期和不定根形成期。用80ppm IBA 喷射母树两周后,个别品种的插穗在离开母树时已分化出不定根原体。插穗母叶内的淀粉和非蛋白氮高,母茎内的碳、氮比高,以及母叶内的蛋白氮含量低的品种,具有较强的发根能力。
The microscopic sections and main biochemical indexes of the rooting process of seven clones of tea clones, including Zhenong 12, were studied. The results showed that the adventitious root primordia originated from the cork thrombosis layer, Pericycle phloem parenchyma cells. According to the anatomical and morphological characteristics of cuttings, the formation of adventitious roots in tea cuttings can be divided into five stages: root undifferentiated stage, root primordium differentiation stage, early stage of root primordium differentiation, late root stage differentiation and adventitious root formation period. Two weeks after the mother tree was sprayed with 80 ppm IBA, the cuttings of individual varieties had differentiated into adventitious roots when they left the mother tree. Starch and non-protein nitrogen in cuttings were high, and the ratio of carbon and nitrogen in stem was high, and the varieties with low protein nitrogen in mother leaves had strong rooting ability.