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用毛细管区带电泳 -电化学检测法同时测定复方芦丁片及果汁中芦丁和 L-抗坏血酸的含量 .研究了电极电位、电解液浓度和酸度、电泳电压及进样时间等对电泳的影响 ,得到了较为优化的测定条件 .以直径为30 0 μm的碳圆盘电极为检测电极 ,电极电位为 1 .0 V(vs.SCE) ,在 2 5 mmol/ L 硼砂 -5 0 mmol/ L Na H2 PO4(p H8.0 )运行缓冲液中 ,上述两组分在 1 2 min内完全分离 .芦丁和 L-抗坏血酸浓度分别在 1 .0× 1 0 - 6～2 .5× 1 0 - 4和 5 .0× 1 0 - 6～ 2 .5× 1 0 - 3 mol/ L 范围内与电泳峰电流呈现良好线性关系 ,检测下限分别为8.0× 1 0 - 7和 3.3× 1 0 - 6mol/ L.9次测定含 5 .0× 1 0 - 5mol/ L 芦丁和 2 .5× 1 0 - 4mol/ L L-抗坏血酸的试样溶液 ,峰高的相对标准偏差分别为 2 .85 %和 1 .6 5 % ,5次测得的平均回收率分别为 97.73%和 99.6 8% .
Simultaneous determination of rutin and L-ascorbic acid in compound rutin tablets and fruit juice by capillary zone electrophoresis with electrochemical detection.The effects of electrode potential, electrolyte concentration and acidity, electrophoresis voltage and injection time on electrophoresis were studied , And the optimal conditions were obtained.Using a carbon disk electrode with a diameter of 30 μm as the detection electrode, the electrode potential was 1.0 V (vs.SCE) Na H2PO4 (p H8.0) running buffer, the above two components were completely separated within 12 min.The concentrations of rutin and L-ascorbic acid were in the range of 1 × 10 6 ~ 2 × 5 - 4 and 5 .0 × 1 0 - 6 ~ 2 .5 × 10 3 mol / L and the peak current of electrophoresis showed a good linearity with the detection limits of 8.0 × 10 -7 and 3.3 × 10 4 - The relative standard deviations (RSDs) of the peak heights of 6mol / L, 9 times, samples containing 5.0 × 10 5 mol / L rutin and 2.5 × 10 4 mol / L L-ascorbic acid were 2.85 % And 1.56% respectively. The average recoveries of five times were 97.73% and 99.6% respectively.