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包涵素~1包裹的细胞膜与真核细胞~2的蛋白运输各种过程密切相关,然而一直还不能直接测试包涵素的功能.文中提出的资料~3证明酿酒酵母在细胞生长和蛋白分泌方面都不需要包涵素.抗酵母包涵素重链的抗血清已被用来从λgt11~4中一群~5酵母DNA中分离出一种分子的重链基因(CHCl)纯系。通过用一种破裂形式的培养生长的纯系DNA以替代单个染色体CHCl的基因而得到缺少包涵素的突变酵母.含有一种无功能CHCl等位基因的细胞不产生免疫反应性多肽重链.,而从变异细胞制成的泡囊缺少包涵素的重链和轻链。虽然缺少色涵素的细胞比正常细胞生长慢2至3倍,但几乎以正常速度分泌转化酶.所以通过分泌途径运偷蛋白不必须配合有包涵素外衣的泡囊的形成.
Inclusion of ~ 1 wrapped cell membrane and eukaryotic cells 2 protein transport process is closely related, however, has not been able to directly test the function of the inclusion protein in the paper presented in ~ 3 to prove that Saccharomyces cerevisiae in cell growth and protein secretion are Antisense Yeast Inclusion Hormone Heavy Chain Antiserum has been used to isolate a molecular heavy chain gene (CHCl) pure line from a group of ~ 5 yeast DNA in lambda gt11 ~ 4. A mutant yeast lacking a cofactor is obtained by replacing a single chromosomal CHCl gene with a propagated, grown, pure line of DNA in a disrupted form Cells that contain a non-functional CHCl allele do not produce immunoreactive polypeptide heavy chains, While vesicles made from variant cells lack the heavy and light chains of the inclusion protein. Although cells lacking the color-rich cells secrete invertase almost at normal speed, 2 to 3 times slower than normal cells, it is not necessary that the secreted protein be transported through the secretory pathway in conjunction with the formation of vesicles that contain the enveloping coat.