论文部分内容阅读
目的:探讨巨噬细胞(M)、肝自然杀伤(NK)细胞在原发性肝细胞癌(HCC)及肝硬化组织中的数量、分布规律及其与患者预后的关系,为HCC患者临床预测提供新思路。方法:应用免疫组化S-P法检测60例HCC,62例单纯性肝硬化,23例正常肝组织M标记物CD68和NK细胞标记物CD57的表达,并分析阳性细胞数量与临床各参数的关系。结果:①各组M平均数从高到低为依次为癌旁组织、肝硬化、正常肝、HCC(P<0.05);NK细胞平均数从高到低依次为HCC、癌旁组织、正常肝、肝硬化(P<0.05)。②HCC中M细胞数量随分化程度降低而减少(P<0.05);NK细胞数量与组织学分级无明显关系。③HCC和癌旁组织中M细胞分布与临床TNM分期无关;HCC中NK细胞随着临床分期的发展有下降的趋势(P<0.05)。④癌组织中15个月内有转移组的M细胞数少于无转移组(P<0.05);15个月内转移复发组癌和癌旁组织的NK细胞计数均明显少于无转移复发组(P<0.01)。⑤在癌组织中M、NK细胞数呈线性正相关(r=0.344,P<0.05)。结论:有转移瘤发生的HCC患者M和NK细胞数量明显少于无转移组;M数量与HCC分化程度密切相关;M和NK细胞计数可能是反映机体抗肿瘤免疫状态和生物学行为以及判断患者预后的重要指标。
Objective: To investigate the relationship between the number and distribution of macrophages (M) and natural killer (NK) cells in primary hepatocellular carcinoma (HCC) and cirrhosis and their relationship with prognosis of patients with HCC Forecast provides new ideas. Methods: The expression of M marker CD68 and NK cell marker CD57 in 60 cases of HCC, 62 cases of simple cirrhosis and 23 cases of normal liver tissue were detected by immunohistochemical SP method. The relationship between the number of positive cells and clinical parameters was analyzed . Results: ① The average number of M in each group was paracancer, cirrhosis, normal liver and HCC from high to low (P <0.05). The average number of NK cells from high to low was HCC, paracancerous tissue, normal Liver, cirrhosis (P <0.05). ② The number of M cells in HCC decreased with the degree of differentiation decreased (P <0.05); There was no significant correlation between the number of NK cells and histological grade. (3) The distribution of M cells in HCC and adjacent tissues was not related to the clinical stage of TNM. The number of NK cells in HCC tended to decline with the development of clinical stage (P <0.05). ④ The number of M cells in the metastasis group within 15 months was less than that in the non-metastasis group (P <0.05). The number of NK cells in the metastasis recurrence group and the adjacent non-cancerous tissue within 15 months were significantly less than those without metastasis Group (P <0.01). ⑤ The number of M and NK cells in cancer tissues was linearly and positively correlated (r = 0.344, P <0.05). Conclusions: The numbers of M and NK cells in HCC patients with metastases are significantly less than those without metastasis. The numbers of M and HCC are closely related to the differentiation degree of HCC. The counts of M and NK cells may reflect the anti-tumor immune status and biological behavior As well as to determine the prognosis of patients with an important indicator.