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目的了解广西梧州市外环境中霍乱弧菌的分布情况。方法采用分离培养法、PCR法对霍乱弧菌进行监测。结果 2009年-2013年广西梧州市外环境中霍乱弧菌的总阳性率为2.80%(56/2 001),2009年最高达6.34%(17/268),2009年-2013年大致呈下降趋势;外环境中水生动物及水样标本中霍乱弧菌的总阳性率分别为3.61%(45/1 246)和1.46%(11/755),两者间差异有统计学意义(χ2=8.02,P<0.01);56株霍乱弧菌包含有4种霍乱菌株:小川型、稻叶型、O139型、非O1非O139型;PCR结果显示,ct和zot均为阴性组有30株,ct阴性zot阳性组共有18株,ct阴性特异基因阳性组共有8株。结论霍乱弧菌可通过水产品的运输而传播,应加强对危险因素中环境水体和食品的监测并制定防控策略。
Objective To understand the distribution of Vibrio cholera in the external environment of Wuzhou in Guangxi. Methods Vibrio cholerae was monitored by isolation and culture method and PCR method. Results The total positive rate of V. cholerae in the external environment of Wuzhou was 2.80% (56/2 001) from 2009 to 2013, reaching as high as 6.34% (17/268) in 2009 and roughly decreasing from 2009 to 2013 ; The total positive rates of V. cholerae in aquatic animals and water samples were 3.61% (45/1 246) and 1.46% (11/755) respectively in external environment, the difference was statistically significant (χ2 = 8.02, P <0.01). Vibrio cholerae contained 4 strains of cholera: Ogawa, Oryza sativa, O139 and non-O1 non-O139. PCR showed that 30 strains were negative for ct and zot, and ct negative There were 18 zot positive groups and 8 ct negative specific gene positive groups. Conclusion Vibrio cholerae can be transmitted through the transport of aquatic products. Monitoring of environmental water bodies and foodstuffs in risk factors should be strengthened and prevention and control strategies should be formulated.