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目的 利用三荧光标记法流式细胞术检测细胞内细胞因子 ,从单细胞水平研究初发系统性红斑狼疮 (SLE)患者Th1/Th2平衡。方法 全血培养后使用荧光标记的抗细胞表面抗原单抗标记表面抗原 ,抗细胞因子单抗进行细胞内染色 ,检测出CD4 + 干扰素 (IFN) γ+ 白细胞介素 (IL) 4 -及CD4 + IFN γ-IL 4 + 细胞作为Th1及Th2细胞 ,从单细胞水平研究初发SLE患者Th1/Th2平衡。结果 未用药治疗的SLE病人其Th1细胞明显低于正常对照组 (P =0 0 2 )。 35例SLE患者按Th1/Th2可分成两组 ,Th1/Th2降低组与Th1/Th2升高组。Th1/Th2降低组其Th1细胞的降低十分明显。Th1/Th2升高组中Th1细胞仅轻度下降。Th1/Th2降低组体现了更高的颊部红斑发生率(P =0 0 36 )。结论 Th1型反应与Th2型反应均参与了SLE的发病。不同患者可能免疫系统的异常并不同。Th1细胞的降低可能与颊部红斑发生有关。
Objective To detect the intracellular cytokines by flow cytometry with three fluorescent markers and to study the Th1 / Th2 balance in patients with newly diagnosed systemic lupus erythematosus (SLE) at the single cell level. Methods Whole blood was cultured and labeled with anti - cytokine monoclonal antibody (McAb) and labeled with fluorescently labeled anti - cell surface antigen (mAb). Intracellular staining of CD4 + IFN - γ + IL - 4 and CD4 + IFN γ-IL 4 + cells as Th1 and Th2 cells to study Th1 / Th2 balance in newly diagnosed SLE patients at the single-cell level. Results Th1 cells in SLE patients without drug treatment were significantly lower than those in the normal control group (P = 0.002). Thirty-five patients with SLE could be divided into two groups according to Th1 / Th2, Th1 / Th2 reduced group and Th1 / Th2 elevated group. Th1 / Th2 decreased group of Th1 cells decreased significantly. Th1 cells decreased only slightly in the Th1 / Th2 elevated group. The Th1 / Th2 reduction group demonstrated a higher incidence of buccal erythema (P = 0 0 36). Conclusion Both Th1-type and Th2-type reactions are involved in the pathogenesis of SLE. Different patients may have different immune system abnormalities. Th1 cell reduction may be related to the occurrence of buccal erythema.