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甘蓝苗期多抗性鉴定是甘蓝多抗性育种的基础，对抗源的筛选有实际应用价值。本项试验，选用４份甘蓝育种自交系材料，１０３，３０２，８４－１０３５－７和９０－１０７２－７。ＴｕＭＶ 选用“甘－８３－８号”毒株，ＣＭＶ选用“甘９１－２３号”毒株，黑腐病菌（Ｘａｎｔｈｏｍｏｎａｓ ｃａｍｐｅｓｔｒｉｓｐｖ．ｃａｍｐｅｓｔｒｉｓ）选用Ｘ_（０－１）菌株。ＴｕＭＶ与ＣＭＶ分别采用人工摩擦的接种方法，黑腐病菌采用喷雾的接种方法，浓度为１０~８个细菌／ｍＬ。试验设有６个接种处理方法①子叶期接种ＴｕＭＶ，第一片真叶接种ＣＭＶ；②子叶期接种ＣＭＶ，第一片真叶接种ＴｕＭＶ；③子叶期一片接种ＴｕＭＶ，另一片接种ＣＭＶ；④子叶期接种ＴｕＭＶ＋ＣＭＶ的混合液；⑤子叶期单接ＣＭＶ；③子叶期单接ＴｕＭＶ；以上６种处理方法分别在５～６片真叶期喷雾接种黑腐病菌。试验３次重复，在防虫温室内进行３个月的时间。接种后温度维持在１０～３０℃，阴天补充光照，待发病后进行抗病性鉴定。试验结果表明第一种接种处理方法，４份试材的发病程度较其它五种都重，另外试材１０３－１，Ａ_（２０），８４－１０３８－１１８６，６０６－１２－２１－１４，８４－１０７２－３－４等自交系较
The identification of multi-resistance in cabbage seedling is the basis of multi-resistant breeding in cabbage, and the selection of resistant sources has practical value. In this experiment, 4 inbred lines of cabbage were selected as materials, 103,302,84-1035-7 and 90-1072-7. TuMV selected “Gan -83-8” strain, CMV selected “Gan 91-23” strain, X. rot (Xanthomonas campestrispv.campestris) selected X_ (0-1) strains. TuMV and CMV were inoculated by artificial rubbing method respectively. The black rot pathogen was sprayed inoculation method at the concentration of 10 ~ 8 bacteria / mL. There were 6 inoculation treatments in the experiment. ① TuMV was inoculated at the cotyledonary stage and CMV was inoculated in the first true leaf; ② CMV was inoculated at the cotyledonary stage, TuMV was inoculated with the first true leaf; ③ TuMV was inoculated in the cotyledon stage and CMV was inoculated with the other one; And inoculated with a mixture of TuMV + CMV; ⑤ single cotyledon CMV; ③ cotyledon TuMV single; more than 6 treatments were sprayed in the 5 to 6 true leaf stage black rot fungi. The experiment was repeated three times and was carried out in the pest control greenhouse for three months. After inoculation, the temperature was maintained at 10 ~ 30 ℃, supplemented by light on the cloudy day, and tested for disease resistance after the onset of disease. The test results showed that the first inoculation method, the four specimens of the disease severity than the other five are heavy, the other test material 103-1, A 20, 84-1038-1186,606-12-21-14, 84-1072-3-4 and other inbred lines