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应用单个细胞克隆化技术，从已建系的人肝癌细胞（ＳＭＭＣ－７７２１）中选育出３个亚系７７２１－ＣＡ，７７２１－ＣＢ，７７２１－ＣＳ（ＣＡ、ＣＢ、ＣＳ），并进行扩大培养，将上述各亚系肝癌细胞接种到裸鼠体内，成瘤率与自发转移率以ＣＳ组最高，ＣＢ组最低。主要的转移器官是肺。原发灶和转移灶肿瘤形态结构一致。ＣＳ细胞形态及其接种的裸鼠移植瘤形态结构和ＳＭＭＣ－７７２１母系细胞基本相似。用ＡＢＣ免疫酶标法检测ＨＢｓＡｇ、ｋｅｒａｔｉｎ、ＡＦＰ、Ｈ－ｒａｓ、Ｃ－ｅｒｂ－２、Ｐ５３癌基因蛋白等肿瘤标记物，ＣＳ组裸鼠移植瘤对Ｈ－ｒａｓ、Ｃ－ｅｒｂ－２癌基因蛋白呈阳性反应，对ＡＦＰ、Ｐ５３呈弱阳性反应，而ＣＢ组裸鼠移植瘤仅对Ｋｅｒａｔｉｎ、Ｃ－ｅｒｂ－２呈弱阳性反应，对其它肿瘤标记物均呈阴性反应。
Using the single cell cloning technique, three sublines, the 7721-CA, 7721-CB, and 7721-CS (CA, CB, and CS), were selected and expanded from the established human hepatoma cells (SMMC-7721). After culture, the above sub-systems of hepatoma cells were inoculated into nude mice. The rate of tumor formation and spontaneous metastasis were highest in the CS group and lowest in the CB group. The main metastatic organ is the lung. The primary tumor and the metastatic tumor have the same morphological structure. The morphology of CS cells and the morphological structure of transplanted tumors in nude mice were similar to those of SMMC-7721 maternal cells. The tumor markers of HBsAg, keratin, AFP, H-ras, C-erb-2, and P53 oncogene proteins were detected by ABC immuno-enzyme immunoassay. The tumors in CS group were transplanted with H-ras and C-erb-2 oncogenes. The protein positive reaction showed a weak positive reaction to AFP and P53, while the CB group had a weakly positive response to Keratin and C-erb-2 in nude mice and negative reaction to other tumor markers.