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比较8种大孔吸附树脂对荭草花旗松素的静态吸附和解吸效果,优化最佳树脂分离纯化荭草花旗松素的工艺技术参数。结果表明:AB-8树脂对荭草花旗松素的吸附和解吸性能较好,对于浓度1.803 mg/mL的花旗松素提取液,AB-8树脂的静态吸附率为61.02%,解吸率94.60%,回收率57.72%;AB-8树脂分离纯化荭草花旗松素的动态吸附和解吸的最佳条件为上柱液浓度1 mg/mL、上柱液流速1 mL/min、洗脱液乙醇的体积分数75%、洗脱液流速1 mL/min、洗脱液用量100 mL;此条件下荭草花旗松素回收率为54.18%,纯度为74.51%。
The adsorption and desorption effects of 8 macroporous adsorption resins on Taxus lepidopteran were compared, and the optimum technical parameters for separating and purifying taxifolium citrinin were optimized. The results showed that the adsorption and desorption properties of Taxus leptin by AB-8 resin were better. The static adsorption rate and the desorption rate of AB-8 resin were 61.02% and 94.60% for the 1.803 mg / mL Taxifolin extract. , And the recovery rate was 57.72%. The optimal conditions for the dynamic adsorption and desorption of Taxus leptin by AB-8 resin were 1 mg / mL on the column, 1 mL / min on the column and ethanol The volume fraction was 75%, the eluent flow rate was 1 mL / min and the eluent volume was 100 mL. Under this condition, the recovery of taxifolin was 54.18% and the purity was 74.51%.