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本试验的结果表明,玉米小斑菌中有的菌株在一对或少数几对同核异质的不育系和保持系上,可以表现为 T 或 C 不育系,比相应的正常细胞质保持系明显感病,而在多对(10对)同核异质的 C 不育系和保持系上却没有发现任何一个菌株能使全部供试的10个 C 不育系都比相应的正常细胞质保持系明显感病。相反,在菌株与寄主的118个组合中,有5%组合的保持系比不育系感病,63.6%个组合的不育系与保持系的病情无明显差异,而不育系比保持系明显感病的组合只占31.4%。这个事实说明,小斑菌中是否真正存在对 C 型雄性不育细胞质专化致病的类群以及由此而提出的根据对雄性不育细胞质的专化鉴别生理小种的方法都需要重新商榷。应该指出,在试验的菌株与寄主的118个组合中,约有1/3组合的 C 不育系比相应的正常细胞质保持系明显感病,这一点在我们今后利用 C 不育系时,即使还未发现 C 小种,也应引起高度重视。
The results of this experiment showed that some of the strains of M.tumefaciens could be expressed as T or C CMS in one pair or a few pairs of isonuclear CMS lines and maintainer lines and maintained above the corresponding normal cytoplasm Were significantly susceptible, whereas none of the 10 CMS lines and maintainer lines found in pairs (10 pairs) of heteronuclear C fertile lines could make all of the 10 CMS lines tested compared with the corresponding normal cytoplasm Maintain the Department of obvious illness. In contrast, among the 118 combinations of strains and hosts, there was no significant difference between the maintainer lines of 5% combination and the maintainer lines of 63.6% Apparently susceptible combination accounted for only 31.4%. This fact indicates that whether there is a real genus of P. monocytogenes in C-type male sterility cytoplasm and the method of identifying the physiological races based on male sterility cytoplasm should be renegotiated. It should be noted that in about 118 combinations of the tested strains and hosts, about one-third of the C-CMS lines were significantly susceptible to the corresponding normal cytoplasmic maintainer lines, which is why, in our future use of CMS lines, C minor species have not yet been found, it should also be highly valued.