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目的探讨应用高效液相色谱仪测定益肾降糖丸中决明子所含橙黄决明素的含量的方法。为建立益肾降糖丸质量标准提供科学依据。方法采用高效液相色谱法,应用Agilent Eelipse XDB-C18色谱柱,以乙腈:0.1%磷酸溶液为流动相进行梯度洗脱,流速:1.0 ml/min,检测波长:284 nm,柱温:40℃,定量测定橙黄决明素含量。结果橙黄决明素在0.0751~0.734μg范围内呈良好的线性关系,平均回收率为100.2%,RSD为0.34%;测定橙黄决明素的含量为每克药品中含决明子1.716 mg,结论该法简便、准确、重现性好,可作为新的决明子含量测定方法。
Objective To study the method of determining the content of cassia toin in cassia seed in Yishen Jiangtang Wan by using high performance liquid chromatography. To establish the Yishen Jiangtang pill quality standards provide a scientific basis. Methods The HPLC elution was performed on an Agilent Eelipse XDB-C18 column with a mobile phase of acetonitrile: 0.1% phosphoric acid. The flow rate was 1.0 ml / min, the detection wavelength was 284 nm, the column temperature was 40 ℃ , Quantitative determination of yellow cassia toxin content. Results The results showed that there was a good linear relationship between the content of the extract of cassia toxins in the range of 0.0751 ~ 0.734μg with the average recovery of 100.2% and RSD of 0.34%. The content of the extract of cassia toxins was 1.716 mg / Simple, accurate, reproducible, and can be used as a new determination of cassia content.