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利用微随体标志和ＰＣＲ，检查６４例宫颈鳞状上皮细胞癌中第三对染色体短臂（３Ｐ）上等位基因的平衡状态，即杂合性缺失（ＬＯＨ）的状况。结果发现５８例为杂合子型的宫颈癌中，４６例（７９％）存在一个或多个标志的ＬＯＨ，其中以３Ｐ１３位点上的ＬＯＨ率为最高，达５２％。联系其临床、病理资料显示，３Ｐ１３的ＬＯＨ率在晚期（临床Ⅲ～Ⅳ期）宫颈癌中为７９％，而在早期（临床Ⅰ～Ⅱ期）癌中仅为４３％，两者差异显著（Ｐ＜０．０５）。但此ＬＯＨ率与肿瘤的病理分级并无显著关系（Ｐ＞０．０５）。结果提示：在第三对染色体短臂上，特别是３Ｐ１３或其附近位点上，可能存在与宫颈癌有关的抑癌基因；而该位点上的ＬＯＨ状况，可能是判断宫颈癌患者预后的一项辅助指标。
Microsatellite markers and PCR were used to examine the balance of the alleles on the third pair of short arm (3P) chromosomes in 64 cases of cervical squamous cell carcinoma, namely the loss of heterozygosity (LOH). The results showed that 58 cases of heterozygous type of cervical cancer, 46 cases (79%) there is one or more markers of LOH, of which the 3P13 locus was the highest rate of up to 52%. According to clinical and pathological data, the rate of LOH in 3P13 was 79% in late stage (clinical stage III to IV) cervical cancer, but only 43% in early stage (clinical stage I to stage II) cancer, and the difference was significant ( P<0.05). However, this LOH rate was not significantly associated with the pathological grade of the tumor (P>0.05). The results suggest that on the short arm of the third pair of chromosomes, especially 3P13 or its nearby sites, tumor suppressor genes associated with cervical cancer may be present; and the LOH status at this site may be used to determine the prognosis of cervical cancer patients. A secondary indicator.