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目的:探讨APC基因杂合性缺失在肾上腺皮质腺瘤中的意义。方法:采用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)方法研究Wnt信号通路成员APC基因11外显子Rsal酶切位点和15外显子MspI酶切位点多态性,分析42例肾上腺皮质腺瘤中APC基因杂合性缺失。结果:在42例肾上腺皮质腺瘤组中杂合子有30例(71.4%),未发现杂合性缺失。结论:肾上腺皮质腺瘤未发现APC基因杂合性缺失现象,据此认为APC基因杂合性缺失在肾上腺皮质腺瘤发生发展过程中可能无明显关联。
Objective: To investigate the significance of loss of APC heterozygosity in adrenocortical adenoma. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to study the polymorphisms of Rsal exon 11 and exon 15 MspI of APC gene in Wnt signaling pathway The heterozygosity of APC gene was analyzed in 42 cases of adrenocortical adenoma. Results: There were 30 heterozygotes (71.4%) in the 42 adrenocortical adenoma groups, and no loss of heterozygosity was found. CONCLUSION: No loss of heterozygosity of APC gene is found in adrenocortical adenoma. Therefore, the loss of heterozygosity of APC gene may not be related to the occurrence and development of adrenocortical adenoma.