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目的以乙型肝炎病毒(HBV)C区为靶位,动物实验体内观察RNA干扰抗HBV的效果。方法以流体动力学法建立HBV感染的动物模型,将pcDNA 3．1-HBV和体外细胞实验证明有效的小干扰 RNA(siRNA)尾静脉共注射BALB／c小鼠;用时间分辨免疫荧光分析法检测小鼠血清中乙型肝炎表面抗原(HBsAg)水平,用荧光定量聚合酶链反应法检测血清HBV DNA水平,用逆转录聚合酶链反应法检测 HBV C-mRNA,用免疫组织化学法检测肝组织HBsAg和乙型肝炎核心抗原。结果在小鼠体内,siRNA 能有效抑制HBV的复制和表达,干扰效果至少持续3 d。结论靶向HBV C区的siRNA在动物体内能有效抗HBV。
Objective To investigate the effect of RNA interference on anti-HBV in vivo in vivo in Hepatitis B virus (HBV) C region. Methods The animal model of HBV infection was established by hydrodynamic method. The BALB / c mice were co-injected with pcDNA3.1-HBV and in vitro experiments. The time-resolved immunofluorescence assay Serum HBV DNA level was detected by fluorescence quantitative polymerase chain reaction (RT-PCR) and HBV C-mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) Tissue HBsAg and hepatitis B core antigen. Results In mice, siRNA can effectively inhibit HBV replication and expression, the interference effect lasting at least 3 d. Conclusion siRNA targeting HBV C region is effective against HBV in animals.