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目的对珠海市2012―2014年登革病毒(DV)进行E基因序列测定,了解其分子病毒学特征,探索其可能的来源。方法采集2012―2014年珠海市登革热流行期间登革热疑似和临床诊断病例的急性期血清样本,采用荧光RT-PCR检测DV核酸,选取50份DV核酸阳性样本采用RT-PCR扩增E基因并进行测序分型。结果 696份血清样本中检出DV核酸阳性152份,阳性率为21.84%;其中DVⅠ型核酸阳性148份,DVⅡ型核酸阳性4份。选取的50份阳性样本全部成功分型,其中46份样本属于DVⅠ型,4份样本属于DVⅡ型。DVⅠ型核苷酸同源性为89.23%~99.93%,推导的氨基酸同源性为97.26%~100.00%,其同源性与2014年广州、2013年中山和2011年印度的DVⅠ型流行株接近。DVⅡ型核苷酸同源性为97.83%~100.00%,推导的氨基酸同源性为97.83%~100.00%,其同源性与2014年印尼、2013年新加坡和2013年深圳的DVⅡ型流行株接近。结论 2012―2014年珠海市DV流行主要以Ⅰ型为主,近几年珠海市DV流行方式属于周边地市或东南亚国家输入性病例引起的本地流行。
Objective To determine the sequence of E gene in dengue virus (DV) from 2012 to 2014 in Zhuhai, and to understand its molecular virological characteristics and explore its possible origin. Methods Acute serum samples of suspected dengue and clinical diagnosis during dengue epidemics in Zhuhai from 2012 to 2014 were collected. Fluorescent RT-PCR was used to detect DV nucleic acid. Fifty DV nucleic acid positive samples were selected for RT-PCR amplification of E gene and sequencing Type. Results A total of 152 positive samples were detected in 696 serum samples, the positive rate was 21.84%. Among them, 148 DV positive samples and 4 DV Ⅱ positive samples. All the 50 positive samples were successfully typed, of which 46 samples belonged to DV Ⅰ type and 4 samples belonged to DV Ⅱ type. The homology of DVⅠ nucleotide was 89.23% -99.93%, and the deduced amino acid homology was 97.26% -100.00%. Its homology was close to those of 2014 epidemic strains in Guangzhou, Zhongshan in 2013 and India in 2011 . The homology of DV Ⅱ nucleotide was 97.83% -100.00%, and the deduced amino acid homology was 97.83% -100.00%. The homology of DV Ⅱ nucleotide nucleotide was close to that of DV Ⅱ strain in 2014 in Indonesia, 2013 in Singapore and 2013 in Shenzhen . Conclusion In 2012-2014, the epidemic of DV in Zhuhai was dominated by type I. In recent years, the epidemic of DV in Zhuhai belonged to the local epidemic caused by imported cases in the surrounding areas or in Southeast Asian countries.