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目的建立同时测定复方环丙沙星滴鼻液中盐酸麻黄碱、乳酸环丙沙星、马来酸氯苯那敏含量的HPLC方法。方法采用:PurospherSTAR RP-18色谱柱(4.6 mm×150 mm,5μm),以磷酸盐缓冲液(p H 3.0±0.1)-乙腈(82∶18)为流动相,调节流速为1.0 ml·min-1,在210 nm波长处同时检测3组分。结果盐酸麻黄碱、乳酸环丙沙星和马来酸氯苯那敏分别在20.14~161.12、12.05~96.38和6.18~49.47μg·ml-1浓度范围内线性关系良好,相关系数分别为0.9996、0.9999和0.9999,平均回收率分别为101.11%、99.83%和101.07%。结论该方法快速简便、重复性、耐用性好,可用于该制剂中3组分的测定。
Objective To establish an HPLC method for simultaneous determination of ephedrine hydrochloride, ciprofloxacin lactate and chlorphenamine maleate in compound ciprofloxacin nasal drops. Methods The Purospher STAR RP RP-18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase consisted of phosphate buffer (p H 3.0 ± 0.1) -acetonitrile (82:18) min-1, at the wavelength of 210 nm at the same time detect 3 components. Results Ephedrine hydrochloride, ciprofloxacin lactate and chlorpheniramine maleate showed good linearity in the range of 20.14-16.112, 12.05-96.38 and 6.18-49.47μg · ml-1, respectively, and the correlation coefficients were 0.9996 and 0.999 And 0.9999 respectively. The average recoveries were 101.11%, 99.83% and 101.07% respectively. Conclusion The method is rapid, simple, reproducible and robust and can be used for the determination of 3 components in this preparation.