[Objective] To extract high quality total RNA from the bark of Eucommia ulmoides Oliv and lay foundation for the isolation of mRNA,cloning of anti-fungi protein gene and library construction of cDNA.[Method] Total RNA from the bark of Eucommia ulmoides Oliv was extracted by modified CTAB-LiCl method,RNApure Plant Kit method and RNAiso Plus method respectively.The quality of total RNA was examined through ultraviolet spectrophotometer and formaldehyde denaturalization agarose gel electrophoresis.[Result] The results showed that total RNA extracted by modified CTAB-LiCl method and RNApure Plant Kit method had higher purity,integrity and yield rate,the ratio of A260/A280 was in the range of 1.8-2.0,the bands of 28S and 18SrRNA were clear;while the quality of total RNA obtained by RNAiso Plus method was poor,the ratio of A260/A280 was 1.652,and the band of RNA was diffusion,which suggested the total RNA was degraded during the extraction process.[Conclusion] The results showed that total RNA from the bark of Eucommia ulmoides Oliv extracted by modified CTAB-LiCl method and RNApure Plant Kit method was of high purity and quality,which could meet the requirement of RT-PCR and RACE etc.experiments as well as lay the foundation for cloning genes from Eucommia ulmoides Oliv. [Objective] To extract high quality total RNA from the bark of Eucommia ulmoides Oliv and lay foundation for the isolation of mRNA, cloning of anti-fungi protein gene and library construction of cDNA. [Method] Total RNA from the bark of Eucommia ulmoides Oliv was extracted by modified CTAB-LiCl method, RNApure Plant Kit method and RNAiso Plus method. The quality of total RNA was examined through ultraviolet spectrophotometer and formaldehyde denaturalization agarose gel electrophoresis. [Result] The results was The results of that total RNA extracted by modified CTAB- The ratio of A260 / A280 was in the range of 1.8-2.0, the bands of 28S and 18SrRNA were clear; while the quality of total RNA was obtained by RNAiso Plus method was poor, the ratio of A260 / A280 was 1.652, and the band of RNA was diffusion, which suggested the total RNA was degraded during the extraction process. [Conclusion] The results showed that total R NA from the bark of Eucommia ulmoides Oliv extracted by modified CTAB-LiCl method and RNApure Plant Kit method was of high purity and quality, which could meet the requirement of RT-PCR and RACE etc. experiments as well as lay the foundation for cloning genes from Eucommia ulmoides Oliv.