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目的:探讨SHH,Notch信号通路和其下游信号分子Gli-1、Hes-1在人子宫内膜癌的表达及意义。方法:应用免疫组织化学方法检测37例正常人子宫内膜、增生内膜和子宫内膜癌中SHH、Notch信号的表达,同时采用RT-PCR方法检测上述新鲜组织中Gli-1、Hes-1的表达。结果:子宫内膜增生组和子宫内膜癌组SHH、Notch的表达均强于正常组(P<0.05)。正常组和子宫内膜增生组Gli-1的表达类似,在子宫内膜癌组表达较低;正常组和子宫内膜增生组Hes-1的表达类似,在子宫内膜癌组表达升高。经随访,本研究选取的病例现均存活。结论:SHH和Notch表达与子宫内膜癌的发生密切相关,Gli-1和Hes-1主要参与子宫内膜癌的进展变化,但可能作用不同。
Objective: To investigate the expression and significance of SHH, Notch signaling pathway and its downstream signaling molecules Gli-1, Hes-1 in human endometrial carcinoma. Methods: Immunohistochemistry was used to detect the expression of SHH and Notch in 37 normal human endometrium, proliferative endometrium and endometrial carcinoma. Meanwhile, the expression of Gli-1, Hes-1 expression. Results: The expressions of SHH and Notch in endometrial hyperplasia group and endometrial carcinoma group were significantly higher than those in normal group (P <0.05). The expression of Gli-1 was similar in normal group and endometrial hyperplasia group, but lower in endometrial carcinoma group. The expression of Hes-1 in normal group and endometrial hyperplasia group was similar, and it was increased in endometrial carcinoma group. After follow-up, the cases selected in this study are now alive. Conclusion: The expression of SHH and Notch are closely related to the occurrence of endometrial carcinoma. Gli-1 and Hes-1 are mainly involved in the progression of endometrial carcinoma, but may play different roles.