论文部分内容阅读
目的:通过体外和体内方法研究β-casomorphin-7对小鼠脾脏淋巴细胞和腹腔巨噬细胞的作用。方法:利用脾细胞增殖试验和腹腔巨噬细胞中NO浓度的变化来研究体外不同的β-casomorphin-7浓度对脾脏淋巴细胞的增殖和腹腔巨噬细胞中NO浓度变化的影响,以及腹腔注射β-casomorphin-7和饮用β-casomorphin-7溶液对上述两个指标的影响。结果:体外试验表明,β-casomorphin-7在不同浓度对脾脏淋巴细胞的增殖显示了刺激和抑制的双向作用,而对NO的产生显示了明显的抑制作用(P<0.01)。体内试验表明,β-casomorphin-7通过腹腔注射和饮用两种给药方式对脾淋巴细胞和腹腔巨噬细胞的作用是一致的。β-casomorphin-7显著地增强了脾淋巴细胞的增殖反应(P<0.01),且抑制了腹腔巨噬细胞NO的产生。结论:当前的试验表明,β-casomorphin-7具有免疫调节作用,且小鼠在2~3周龄时,可吸收入血发挥免疫调节作用。
OBJECTIVE: To study the effect of β-casomorphin-7 on splenic lymphocytes and peritoneal macrophages in vitro and in vivo. Methods: The effects of different concentration of β-casomorphin-7 in vitro on the proliferation of splenic lymphocytes and NO concentration in peritoneal macrophages were studied by using spleen cell proliferation assay and NO concentration in peritoneal macrophages. -casomorphin-7 and drinking β-casomorphin-7 solution on the above two indicators. RESULTS: In vitro experiments showed that β-casomorphin-7 showed a two-dimensional effect of stimulation and inhibition on proliferation of splenic lymphocytes at various concentrations, but showed a significant inhibitory effect on the production of NO (P <0.01). In vivo experiments showed that the effect of β-casomorphin-7 on splenic lymphocytes and peritoneal macrophages by intraperitoneal injection and drinking were consistent. β-casomorphin-7 significantly enhanced the proliferation of splenic lymphocytes (P <0.01), and inhibited the production of NO in peritoneal macrophages. CONCLUSIONS: Current tests indicate that β-casomorphin-7 has an immunomodulatory effect and that mice can uptake into the blood at 2 to 3 weeks of age to play an immunomodulatory role.