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目的:探讨间充质干细胞(marrow mesenchymal stem cells,MSCs)对RM-1小鼠前列腺癌细胞生长的影响及其可能机制。方法:从小鼠骨髓中无菌分离培养扩增MSCs。将MSCs与RM-1细胞混合后接种于Balb/c小鼠皮下,观察肿瘤生长情况;采用免疫组化法检测肿瘤组织血管生成情况(CD34染色);采用RealtimePCR法和ELISA法检测MSCs促进血管生成相关分子的表达情况。结果:MSCs与RM-1小鼠前列腺癌细胞混合后接种于Balb/c小鼠皮下,与对照组相比,MSCs可明显促进肿瘤生长(P<0.05);前列腺癌组织中CD34免疫组化染色结果证实MSCs组较对照组微血管密度显著升高(P<0.05);Realtime PCR及ELISA结果显示MSCs条件培养上清中VEGF表达显著升高。结论:MSCs可能通过表达VEGF促进前列腺癌血管形成,从而达到促进肿瘤生长的目的。
Objective: To investigate the effect of mesenchymal stem cells (MSCs) on the growth of prostate cancer cells in RM-1 mice and its possible mechanism. Methods: Aseptic MSCs were isolated from mouse bone marrow. The MSCs were mixed with RM-1 cells and inoculated into Balb / c mice subcutaneously to observe the growth of the tumor. Immunohistochemistry was used to detect the tumor angiogenesis (CD34 staining). MSCs were stimulated by Realtime PCR and ELISA Related molecules expression. Results: MSCs were inoculated into Balb / c mice subcutaneously after being mixed with RM-1 mouse prostate cancer cells. Compared with the control group, MSCs could significantly promote tumor growth (P <0.05); CD34 immunohistochemical staining The results confirmed that the microvessel density of MSCs group was significantly higher than that of the control group (P <0.05). Realtime PCR and ELISA showed that the expression of VEGF in supernatant of MSCs was significantly increased. Conclusion: MSCs may promote the formation of prostate cancer by VEGF expression, so as to achieve the purpose of promoting tumor growth.