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目的:通过检测宫颈癌患者中HPV-16的患病率和不同级别宫颈病变中HPV存在状态的研究探讨HPV-16整合在宫颈癌发生发展中的作用。方法:运用HPV通用型引物及HPV-16型特异型引物相继检测宫颈癌标本中HPV-16感染的患病率,采用定量PCR的方法检测HPV-16感染的112例宫颈癌标本、151例CINI标本、246例CINⅡ标本和120例CINIII标本中HPV的存在状态,分析HPV-16整合状态与宫颈病变严重程度的相关性。结果:在宫颈癌患者中HPV-16感染患者有112例,约占宫颈癌总患者数的56.57%;在112例感染HPV-16的宫颈癌标本中,HPV-16单纯整合感染的病例超过半数共有73例,阳性率达到65.18%(73/112);在CINI、CINⅡ、CIN III患者中,HPV-16单纯整合型感染率分别为7.95%、23.58%和39.17%,宫颈癌患者的HPV-16整合率与CINI、CINⅡ、CINIII患者相比差异有统计学意义。结论:HPV-16是宫颈癌患者中最常见的病毒感染类型,在感染HPV-16的患者中HPV的整合状态与宫颈病变严重程度明显呈正相关。如能在HC-ⅡHPVDNA检测的基础上辅以HPV的分型检查及病毒整合状态的检测将更加有利于宫颈癌前病变患者的预后判断和增加宫颈癌筛查的准确性。
Objective: To investigate the role of HPV-16 in the development of cervical cancer by detecting the prevalence of HPV-16 in cervical cancer patients and the status of HPV in different grades of cervical lesions. Methods: The prevalence of HPV-16 infection in cervical cancer specimens was detected by using HPV universal primer and HPV-16 specific primer. 112 cervical cancer samples with HPV-16 infection and 151 CINI samples were detected by quantitative PCR 246 cases of CIN Ⅱ specimens and 120 cases of CINIII specimens of HPV status, analysis of HPV-16 integration status and the severity of cervical lesions. Results: There were 112 cases of HPV-16 infection in cervical cancer patients, accounting for 56.57% of the total number of cervical cancer cases. Of the 112 HPV-16-positive cervical cancer samples, more than half The positive rate of HPV-16 infection was 7.95%, 23.58% and 39.17% in CINI, CINⅡ and CIN III patients, respectively. The positive rates of HPV- 16 The integration rate and CINI, CIN Ⅱ, CINIII patients compared with the difference was statistically significant. Conclusion: HPV-16 is the most common type of viral infection in patients with cervical cancer. The positive correlation between the HPV status and the severity of cervical lesions in HPV-16-infected patients is obviously positive. If HC-Ⅱ HPV DNA test combined with HPV typing and virus integration status of detection will be more conducive to prognosis of cervical precancerous lesions and increase the accuracy of cervical cancer screening.