论文部分内容阅读
目的评价富硒和转白细胞介素2(IL2)基因双歧杆菌对小鼠移植瘤H22的抑制效果。方法通过电转导将含IL2质粒导入到长双歧杆菌(Bifidobacterium longum,B.longum)中,将转IL2基因双歧杆菌接种到添加了亚硒酸钠(Na2SeO3)的培养基中,利用微生物的富集及转化作用,形成富硒的含IL2基因质粒的双歧杆菌(Se-B.longum-IL2)。结果利用双歧杆菌的肿瘤厌氧区靶向性,通过荷肝癌H22的小鼠尾静脉注射Se-B.longum-IL2,取得了良好的抑瘤效果与荷肝癌小鼠生存延长效果。结论转IL2基因双歧杆菌和富硒联合后对小鼠肝癌有明显的基因治疗前景。
Objective To evaluate the inhibitory effect of selenium and interleukin 2 (IL2) gene Bifidobacterium on mice transplanted tumor H22. Methods The IL2 containing plasmid was introduced into Bifidobacterium longum (B. longum) by electrotransduction. The Bifidobacterium that translocated IL2 gene was inoculated into the medium supplemented with Na2SeO3, Enriched and transformed into Se-B.longum-IL2, a selenium-enriched IL2 gene-containing plasmid. Results The anaerobic targeting of Bifidobacterium was used in the study. Se-B.longum-IL2 was injected into the caudal vein of H22-bearing mice to obtain a good anti-tumor effect and to prolong the survival of hepatoma-bearing mice. Conclusion The transgenic Bifidobacterium IL2 gene and selenium-rich combination of mouse liver cancer have obvious prospects for gene therapy.