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利用酵母双杂合体系统，检测与植物线状病毒组中热休克蛋白ＨＳＰ７０相结合的其它相关的ＢＹＶ蛋白。在体外构建两种编码融合蛋白的酵母表达质粒：一组为将编码转录激活区域的基因与ＨＳＰ７０ｒ基因融合并克隆到ｐＡＣＴ质粒中（ｐＡＣＴ·ＨＳＰ７０ｒ），它编码转录激活区域与ＨＳＰ７０蛋白的融合蛋白；另一组为将编码ＤＮＡ结合区域的基因分别与甜菜黄化病毒基因组其它７个基因融合并克隆到ｐＡＳ质粒中（ｐＡＳ·－），分别编码７个融合蛋白。经两质粒共转化酵母细胞后，通过双报告基因（Ｌａｃｚ基因和组氨酸Ｈｉｓ３基因）筛选获得阳性菌落，以阳性菌落总ＤＮＡ为模板，经体外特异性引物的ＰＣＲ扩增，检测出ＨＳＰ７０蛋白与ｐ２０蛋白之间的相互作用，并证实这一相互作用是特异的。
Using the yeast two-hybrid system, other related BYV proteins that bind to the heat shock protein HSP70 in the plant linear virus group were tested. Two yeast expression plasmids encoding the fusion protein were constructed in vitro: One was a fusion of the gene encoding the transcriptional activation region with the HSP70r gene and cloning into the pACT plasmid (pACT · HSP70r), which encodes a fusion protein of transcriptional activation region and HSP70 protein ; The other group was fused to the other seven genes of the beet genome and cloned into the pAS plasmid (pAS · -), respectively encoding seven fusion proteins. After the two plasmids were transformed into yeast cells, positive colonies were screened by double reporter genes (Lacz gene and histidine His3 gene). The positive colonies were used as template to amplify the HSP70 protein by PCR with in vitro specific primers And p20 protein interactions, and confirmed that this interaction is specific.