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为探讨免疫反应在丁型肝炎发病中的作用，采用碱性磷酸酶抗碱性磷酸酶（ＡＰＡＡＰ）免疫酶染色技术，观察比较了３０例乙型肝炎病毒（ＨＢＶ）合并丁型肝炎病毒（ＨＤＶ）感染患者和３０例单纯ＨＢＶ感染者外周血及肝组织中的细胞免疫状态。结果发现ＨＢＶ合并ＨＤＶ感染者外周血ＣＤ８＋Ｔ细胞和ＣＤ１６＋细胞数显著高于单纯ＨＢＶ感染者，而与ＨＤＶ感染后是否抑制ＨＢＶ复制无关。在感染肝组织中，汇管区浸润细胞以ＣＤ４＋Ｔ细胞为主，小叶内坏死区中以ＣＤ８＋Ｔ细胞为主，与单纯ＨＢＶ感染者比较，两者差异无显著性。表明Ｔ细胞所介导的免疫应答在ＨＤＶ感染后所引起的肝细胞损伤中发挥着重要作用。
In order to explore the role of immune response in the pathogenesis of hepatitis D, the alkaline phosphatase-resistant alkaline phosphatase (APAAP) immunoenzymatic staining technique was used to observe the effects of hepatitis B virus (HBV) combined with hepatitis D virus (HDV ) Infected patients and 30 patients with HBV infection in peripheral blood and liver tissue in the cellular immune status. The results showed that the number of CD8 + T cells and CD16 + cells in peripheral blood of patients with HBV infection complicated with HDV was significantly higher than that of patients with HBV infection alone, but not with HDV infection. In the infected liver tissue, the infiltrating cells in the portal area mainly were CD4 + T cells, while the CD8 + T cells were predominant in the necrotic area of lobular cells. Compared with HBV infected patients, there was no significant difference between the two groups. Suggesting that T cell mediated immune responses play an important role in hepatocellular injury induced by HDV infection.