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目的了解2016年北京地区急性腹泻患儿感染人博卡病毒(HBoV)流行及遗传进化特征。方法采集北京地区哨点医院2016年<60月龄急性腹泻患儿粪便标本,用巢式PCR检测HBoV,通过测序和Blast比对确定病毒型别,并进行遗传进化分析;采用实时荧光PCR对A组轮状病毒、诺如病毒、肠道腺病毒、星状病毒进行检测,分析混合感染情况。结果 354份粪便标本中,HBoV阳性26份,阳性率为7.34%,其感染无明显季节分布,<2岁患儿占84.62%。HBoV与其他腹泻病毒混合感染率为38.46%,以混合轮状病毒感染为主。进化分析表明,HBoV1、2、3型感染分别为15、10、1例,未检出HBoV4型。HBoV2序列均独立于HBoV2A和HBoV2B亚型分支,位于一个新的HBoV2亚型分支上。结论 2016年北京地区腹泻患儿感染HBoV包括3种型别(1-3型),2岁以内患儿是高发人群。HBoV2在进化树上位于一个新的亚型分支。
Objective To investigate the epidemiology and genetic evolution of human Boca inactivated virus (HBoV) in children with acute diarrhea in Beijing in 2016. Methods Sentinel samples of children with acute diarrhea <60 months were collected from sentinel hospital in Beijing in 2016, nested PCR was used to detect HBoV, the virus type was determined by sequencing and Blast analysis, and the genetic evolution was analyzed. Real- Group rotavirus, norovirus, enteric adenovirus, astrovirus detection, analysis of mixed infections. Results Of the 354 stool samples, 26 were positive for HBoV, the positive rate was 7.34%. There was no obvious seasonal distribution of infection, 84.62% of children <2 years old. The mixed infection rate of HBoV with other diarrhea virus was 38.46%, mainly with mixed rotavirus infection. Phylogenetic analysis showed that HBoV1, 2, and 3 infections were 15, 10, and 1 cases, respectively. HBoV4 was not detected. The HBoV2 sequences are all independent of the HBoV2A and HBoV2B subtypes and are located on a new HBoV2 subtype. Conclusions HBeV infection in children with diarrhea in Beijing in 2016 includes 3 types (1-3 type) and children within 2 years of age are high risk population. HBoV2 is on a new subtype branch in the evolutionary tree.