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目的:观察血尿安片对IgA肾病(IgAN)大鼠补体调节蛋白CR1的影响。方法:36只SD大鼠随机分为对照组、模型组、血尿安片组,建立IgAN模型。成模后血尿安片组从第10~12周给予血尿安片7.0 g·kg~(-1)灌胃,qd;对照组和模型组以生理盐水灌胃。检测各组大鼠第10周、11周、12周时24h尿蛋白定量、第12周末各组大鼠红细胞CR1,观察大鼠肾脏病理结构,免疫组化检测大鼠肾脏组织的CR1的表达。结果:与模型组比较,血尿安片组大鼠的24 h蛋白尿定量明显降低(P<0.05),红细胞CR1数量明显增加(P<0.05),肾组织中的CR1表达上调,肾组织的损伤较轻。结论:血尿安片可能通过调控CR1来干预IgAN的补体活化,从而治疗IgAN。
Objective: To observe the effect of Xueju An Tablets on complement regulatory protein CR1 in IgA nephropathy rats. Methods: Thirty-six SD rats were randomly divided into control group, model group and Xueju An Pian group. IgAN model was established. After the model was established, hematuria tablets group was given gurlitang 7.0 g · kg ~ (-1) gavage from the 10th to 12th week, qd; the control group and the model group were given gavage with saline. The urinary protein excretion was measured at the 10th week, the 11th week and the 12th week in each group. At the end of the 12th week, the CR1 level of erythrocytes in each group was observed. The pathological structure of kidney was observed. The expression of CR1 was detected by immunohistochemistry. Results: Compared with the model group, 24 h urinary protein excretion was significantly decreased (P <0.05), the number of CR1 in erythrocytes was significantly increased (P <0.05), the expression of CR1 in renal tissue was up-regulated, and the damage of renal tissue Lighter. Conclusion: Xuehui Anypian can interfere with complement activation of IgAN by regulating CR1 to treat IgAN.