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目的:研究大鼠禁食状态下甲状腺内胃动素(MTL)对胃肠移行性复合运动(MMC)的影响。方法:采用免疫荧光法、放射免疫法检测MTL在甲状腺组织的表达;采用清醒状态下胃肠运动记录法观察甲状腺内MTL对MMC的调控作用。结果:免疫荧光结果显示,正常甲状腺组织中有MTL免疫阳性反应物(MTL-IR)的表达,且MTL-IR与降钙素(CT)共存,提示MTL表达于甲状腺的C细胞。放射免疫结果显示,十二指肠黏膜有大量MTL表达,其含量分别是甲状腺和胃窦部MTL表达的128.02和100.57倍。大鼠尾静脉注射MTL,胃窦和十二指肠的MMC呈剂量依赖性增加,表现为收缩频率增快(P<0.05,P<0.01)和收缩波幅增大(P<0.05,P<0.01)。静脉注射MTL受体拮抗剂GM109,MMC收缩频率显著减慢(P<0.05),收缩波幅显著变小(P<0.05);GM109可显著阻断MTL的促进MMC作用。摘除一侧甲状腺,MMC周期显著变长,收缩频率显著减慢(P<0.05),收缩波幅显著变小(P<0.05)。甲状腺内注射MTL抗血清,胃窦和十二指肠的MMC运动均显著减少,其收缩频率显著减慢(P<0.05),收缩波幅显著变小(P<0.05)。结论:甲状腺C细胞可表达MTL;甲状腺内MTL参与胃肠MMC的调控。
AIM: To investigate the effect of intrathyroid motilin (MTL) on gastrointestinal migrating complex movement (MMC) under fasting conditions in rats. Methods: The expression of MTL in thyroid tissue was detected by immunofluorescence and radioimmunoassay. The regulation of MTL in thyrocytes on MMC was observed by gastrointestinal motility recording in awake state. Results: The results of immunofluorescence showed that the expression of MTL-IR in normal thyroid tissue and MTL-IR coexisted with calcitonin (CT), suggesting that MTL was expressed in thyroid C-cells. The results of radioimmunoassay showed that there was a large amount of MTL expression in duodenal mucosa, which was 128.02 and 100.57 times of MTL in thyroid and gastric antrum, respectively. In the tail vein of rats, MTL injection, MMC in gastric antrum and duodenum increased in a dose-dependent manner. The systolic frequency was increased (P <0.05, P <0.01) and systolic amplitude was increased ). The systolic frequency of GM109 and MMC decreased significantly (P <0.05) and the amplitude of contraction decreased significantly (P <0.05). GM109 could significantly inhibit the effect of MTL on MMC. Excision of the thyroid gland at one side significantly prolonged the MMC cycle, significantly reduced the frequency of contractions (P <0.05) and decreased the amplitude of contractions (P <0.05). Intra-thyroid injection of MTL antiserum, gastric antrum and duodenum significantly reduced MMC movement, the contraction frequency was significantly slowed down (P <0.05), contraction amplitude was significantly smaller (P <0.05). CONCLUSION: MTL can express MTL in thyrocytes and MTL in thyroid is involved in the regulation of gastrointestinal MMC.