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目的建立HPLC同时测定芫花药材中伞形花内酯、芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-吡喃葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素及芫花素含量的方法。方法色谱柱:Diamonsil C18(250 mm×4.6 mm5,μm),流动相:甲醇(A)-体积分数为0.8%的醋酸水溶液(B),梯度洗脱(0～15 min:45%A、15～20 min:45%A～51%A、20～40 min:51%A、40～42 min:51%A～82%A4、2～60 min:82%A),流速:0.8 mL·min-1,波长:338 nm。结果伞形花内酯、芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-吡喃葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素、芫花素的质量浓度依次分别在0.22～1.78、0.47～3.77、0.10～0.76、0.22～1.72、0.49～3.94、0.86～6.84、0.88～7.040、.44～3.56 mg·L-1内与峰面积呈良好的线性关系,平均回收率分别为98.7%、98.1%、97.4%、97.7%、101.1%、101.7%、100.3%、98.7%、RSD分别为2.7%、1.0%、2.4%、1.3%1、.8%、1.2%、1.6%2、.2%(n=5)。结论该法可为芫花药材的质量控制和临床用药安全提供依据。
OBJECTIVE To establish an HPLC method for simultaneous determination of ursovandin, 5-O-β-D-cinnamaldehyde glycoside and 5-O-β-D-glucopyranoside in Daphne genkwa, Luteolin, lincoside, apigenin, hydroxy genkwanin and genkwanin content of the method. Methods The mobile phase consisted of Diamonsil C18 (250 mm × 4.6 mm 5, μm), mobile phase of methanol (A) - acetic acid solution with a volume fraction of 0.8%, gradient elution (0-15 min: 45% A, 15 20 min: 45% A to 51% A, 20 to 40 min: 51% A, 40 to 42 min: 51% A to 82% A4, 2 to 60 min: 82% A), flow rate: 0.8 mL · min -1, wavelength: 338 nm. Results Umbelliferone, Dacoxacin-5-O-β-D-cinnamaldehyde glycoside, Dactylisin-5-O-β-D-glucopyranoside, luteolin, Hydroxy genkwanin, genkwanin in the order of 0.22 ~ 1.78,0.47 ~ 3.77,0.10 ~ 0.76,0.22 ~ 1.72,0.49 ~ 3.94,0.86 ~ 6.84,0.88 ~ 7.040, .44 ~ 3.56 mg · The average recoveries were 98.7%, 98.1%, 97.4%, 97.7%, 101.1%, 101.7%, 100.3% and 98.7%, respectively, with a RSD of 2.7% and 1.0%, respectively. , 2.4%, 1.3% 1, .8%, 1.2%, 1.6% 2, .2% (n = 5). Conclusion The method can provide the basis for quality control and clinical drug safety of Daphne genkwa.