【摘 要】
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Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain.The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2C glycoprotein (SAD-
【机 构】
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Shenzhen Key Lab of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Scienc
论文部分内容阅读
Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain.The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2C glycoprotein (SAD-RV(△G)-N2C(G)) shows strong neuro-tropism in cell culture,but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2C(G) and two other widely-used retrograde tracers,SAD-RV(△G)-B19(G) and rAAV2-retro.We found that SAD-RV(△G)-N2C(G) enhanced the infection efficiency of long-projecting neurons by ～ 10 times but with very similar neuro-tropism,compared with SAD-RV(△G)-B19(G).On the other hand,SAD-RV(△G)-N2C(G) had an infection efficiency comparable with rAAV2-retro,but a more restricted diffusion range,and broader tropism to different types and regions of long-projecting neuronal populations.These results demonstrate that SAD-RV(△G)-N2C(G) can serve as an effective retrograde vector for studying neuronal circuits.
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