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背景:研究表明,亚低温和适当升压对脑缺血都有一定的保护作用,二者联合应用可能产生协同作用,起到更好的脑保护效果。目的:通过升压联合亚低温对大鼠局灶性脑缺血再灌注后脑梗死体积、血脑屏障的影响,探讨脑保护作用机制。设计:以实验动物为研究对象,完全随机对照设计。单位:两所大学医院的神经科和一所市级医院的皮肤科。材料:实验于2001-03/07在华中科技大学同济医学院附属协和医院神经科实验室和武汉大学人民医院神经科实验室完成。选择64只成年Wistar大鼠由武汉大学人民医院实验动物中心提供。干预:制备大鼠局灶性脑缺血模型,随机分为对照组、升压组、亚低温组及升压联合亚低温治疗组(联合组),每组16只动物,缺血3h再灌注;对照组常温不予处理,升压组缺血2h给予升压处理3h,亚低温组缺血2h开始亚低温干预,联合组联合应用亚低温组和升压组治疗方法。缺血24h处死动物。主要观察指标:神经功能缺失评分、脑梗死体积和血脑屏障破坏情况。结果:升压组、亚低温组及联合组的神经功能缺失评分分别为(2.12±0.54)分、(2.14±0.69)分、(1.78±0.61)分,脑梗死体积分别为(17.65±4.78)%,(16.21±3.79)%,(11.31±3.64)%,Even’s蓝染色体积分别为(56.63±10.70)mm3,(53.52±8.44)mm3,(38.45±5.25)mm3均明显低于对照组?
Background: Studies have shown that mild hypothermia and appropriate boost have some protective effect on cerebral ischemia, the combination of the two may have a synergistic effect, play a better brain protection effect. OBJECTIVE: To explore the mechanism of cerebral protection by increasing the pressure combined with mild hypothermia on the volume of cerebral infarction and the blood-brain barrier after focal cerebral ischemia-reperfusion in rats. Design: The experimental animals as the research object, a completely randomized controlled design. Unit: Neurology at two University Hospitals and dermatology at a municipal hospital. MATERIALS: Experiments were performed at the Neurology Laboratory of Peking Union Medical College Hospital, Huazhong University of Science and Technology and the Neurology Laboratory of Wuhan University People’s Hospital from March to July in 2001. Sixty-four adult Wistar rats were selected from Experimental Animal Center of Wuhan University People’s Hospital. Intervention: The model of focal cerebral ischemia in rats was established and randomly divided into control group, booster group, hypothermia group and booster combined with mild hypothermia treatment group (combination group), 16 animals in each group, ischemia and reperfusion ; The control group was not treated at room temperature, the blood pressure group 2h was treated with blood pressure for 3h, the mild hypothermia group 2h began mild hypothermia intervention, the combination group combined with mild hypothermia and the treatment group. Animals were sacrificed 24h after ischemia. MAIN OUTCOME MEASURES: Neurological deficit score, volume of cerebral infarction, and destruction of the blood-brain barrier. Results: The scores of neurological deficit in the booster group, the hypothermia group and the combined group were (2.12 ± 0.54) points, (2.14 ± 0.69) points and (1.78 ± 0.61) points respectively, and the infarct volume were (17.65 ± 4.78) %, (16.21 ± 3.79)% and (11.31 ± 3.64)%, respectively. Even blue staining volume was (56.63 ± 10.70) mm3, (53.52 ± 8.44) mm3 and (38.45 ± 5.25) mm3 were significantly lower than those in the control group.