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利用ＤＮＡ 对Ｒｕ（ｂｉｐｙ）２ （ＰＩＰ）（Ⅱ）（结构见图１）的荧光增强作用建立了测定ＤＮＡ 的新方法。激发，发射波长分别为４６９ ｎｍ 和５９０ ｎｍ ，当Ｒｕ（ｂｉｐｙ）２ＰＩＰ（Ⅱ）的浓度为１．０ μｇ／ｍ Ｌ时，体系的荧光强度达到最大。在选择的最佳实验条件下，体系的荧光强度与小牛胸腺（ＣＴ）ＤＮＡ 的浓度在０～１．２５ μｇ／ｍ Ｌ的范围内呈良好的线性关系。方法的检出限为１１．２ ｎｇ／ｍ Ｌ。对０．５ μｇ／ｍ ＬＣＴ ＤＮＡ 平行测定１１ 次的相对标准偏差为１．２％ 。将该方法用于合成样品的分析，获得满意结果
A new method for the determination of DNA was established using the fluorescence enhancement of Ru (bipy) 2 (PIP) (Ⅱ) (see Figure 1) for DNA. The excitation and emission wavelengths were 469 nm and 590 nm, respectively. When the concentration of Ru (bipy) 2PIP (Ⅱ) was 1.0 μg / m L, the fluorescence intensity reached the maximum. Under the optimal experimental conditions, the fluorescence intensity of the system showed a good linear relationship with the concentration of DNA of calf thymus (CT) in the range of 0 ~ 1.25 μg / mL. The detection limit of the method was 11.2 ng / m L. The relative standard deviation of 11 replicates with 0.5 μg / mL LCT DNA was 1.2%. The method was applied to the analysis of synthetic samples to obtain satisfactory results