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目的研究浙江地区住院治疗的炎症性肠病(IBD)患者的可能危险因素及NOD2/CARD15基因3个单核苷酸多态性(SNPs)与IBD的相关性。方法通过问卷调查对2005年1月至2008年12月浙江大学医学院附属第一医院、浙江省金华市中心医院、宁波李惠利医院及台州地区医院136例IBD患者和等量健康对照者进行病例对照研究,对结果进行COX回归分析;采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)法对60例无血缘关系的IBD患者及60例健康对照者NOD2/CARD15基因Arg702Trp、Gly908Arg和Leu1007fsinsC多态性进行分析。结果 IBD平均发病年龄42.6岁,男性稍多于女性。牛奶、油炸食品和精神因素在IBD致病因素中具有统计学意义。IBD患者和健康对照者均未检测出纯合子和杂合子的单核苷酸多态性(SNPs)突变。结论牛奶、油炸食品和精神紧张可能是浙江地区IBD潜在的危险因素,白种人克罗恩病(CD)患者相关易感基因NOD2/CARD15常见的3个SNPs与浙江地区IBD患者无关。
Objective To investigate the possible risk factors of inpatient treatment of inflammatory bowel disease (IBD) and the association of 3 SNPs of NOD2 / CARD15 gene with IBD in Zhejiang province. Methods From January 2005 to December 2008, 136 cases of IBD patients and equivalent healthy controls from the First Affiliated Hospital of Zhejiang University Medical School, Jinhua Central Hospital of Zhejiang Province, Ningbo Li Huili Hospital and Taizhou Regional Hospital were enrolled in this study. The results of COX regression analysis; PCR-RFLP analysis of 60 cases of unrelated IBD patients and 60 healthy controls NOD2 / CARD15 gene Arg702Trp , Gly908Arg and Leu1007fsinsC polymorphisms were analyzed. Results The average age of onset of IBD was 42.6 years old, slightly more men than women. Milk, fried foods and mental factors were statistically significant in the pathogenesis of IBD. Neither homozygous nor heterozygous SNPs were detected in both IBD patients and healthy controls. Conclusion Milk, fried food and mental stress may be the potential risk factors for IBD in Zhejiang Province. The three common SNPs of NOD2 / CARD15 associated with susceptibility gene in Caucasian Crohn ’s disease (CD) patients are not related to IBD patients in Zhejiang Province.