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目的 :建立测定血浆中异烟肼浓度的高效液相色谱法。方法 :色谱柱 :EclipseXDBC18(4 6mm×150mm ,5μm ) ;流动相 :0 05mol/L磷酸二氢铵 -乙腈 (65∶35)。0 5ml血浆用10 %三氯醋酸沉淀蛋白后 ,加桂皮醛衍生化 ,再用乙醚提取分离后进样。结果 :在0 10～12 0μg/ml范围内线性关系良好 ;日内误差小于7 % ,日间误差小于14 % ;方法平均回收率95 %～105 %。结论 :本方法具有快捷、灵敏度较高、干扰少等特点 ,可用于异烟肼的药代动力学研究
Objective: To establish a HPLC method for the determination of isoniazid in plasma. Methods: Column: EclipseXDBC18 (4 6mm × 150mm, 5μm); mobile phase: 0 05mol / L ammonium dihydrogen phosphate - acetonitrile (65:35). 0 5ml plasma with 10% trichloroacetic acid precipitated protein, cinnamic aldehyde derivatization, and then extracted with ether injection. Results: The linearity was good in the range of 0 10 ~ 12 0μg / ml. The intra-day error was less than 7% and the daytime error was less than 14%. The average recovery was 95% -105%. Conclusion: The method is fast, sensitive and less interference, can be used for the pharmacokinetics of isoniazid