论文部分内容阅读
采用植物组织培养技术,利用驱蚊草顶芽及带腋芽茎段为外植体,开展驱蚊草的诱导分化、芽苗增殖、壮苗培养、生根和移栽等系列研究。结果表明,采用二次消毒方法,有利于无菌系的建立;在无菌系建立后,以带叶柄叶片为材料,在MS+BA0.5mg/L+NAA0.1mg/L培养基上进行培养,可分化、获得高达60%的正常丛生芽;以1/2MS+6-BA0.1mg/L+NAA0.1mg/L为丛生芽增殖继代培养基,不仅能够满足快繁、增殖,同时也能够降低驱蚊草试管苗玻璃比发生频率;生根培养基用1/2MS+6-BA0.1mg/L+NAA0.5mg/L外加AC0.5mg/L,炼苗后将瓶苗移栽到泥炭土、沙、蛭石(质量比为2:3:1)的基质中,控温、保温、保湿栽,成活率可达80%左右。
The plant tissue culture technique was used to study the inducement and differentiation, the proliferation of sprouting seedling, the seedling culture, rooting and transplanting of insect repellent grass by using insect repellent grass buds and axillary buds as explants. The results showed that the establishment of asepsis was facilitated by the secondary disinfection method. After the establishment of the asexual line, the petioles were cultured on MS + BA0.5mg / L + NAA0.1mg / L medium , Can be differentiated, up to 60% of the normal cluster buds; 1 / 2MS + 6-BA0.1mg / L + NAA0.1mg / L as a cluster of bud proliferation subculture medium, not only to meet the rapid propagation and proliferation, but also Can reduce the frequency of the glass ratio of repellent grass tube seedlings; rooting medium with 1 / 2MS + 6-BA0.1mg / L + NAA0.5mg / L plus AC0.5mg / L, after the bottle seedlings transplanted to peat soil, Sand, vermiculite (mass ratio of 2: 3: 1) matrix, temperature control, heat preservation, moisture planted, the survival rate of up to 80%.