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目的:考察木蹄复方提取物(ECF)的体内抗肿瘤活性,并探讨部分作用机制。方法:制备S 180和Lewis肺癌实体瘤小鼠模型,接种次日将2种实体瘤模型小鼠均分为模型组,EFC低、中、高剂量(0.05,0.1,0.15 g.kg-1)组和环磷酰胺(CTX)阳性对照组,分别连续给药8和15 d,观察ECF对荷瘤小鼠瘤质量、胸腺指数和脾脏指数、血清中肿瘤坏死因子-α(TNF-α)和巨噬细胞集落刺激因子(M-CSF)水平的影响。结果:ECF低、中、高3个剂量组对S 180荷瘤小鼠肿瘤抑制率分别为11.8%,47.1%和58.8%;对Lewis肺癌实体瘤小鼠肿瘤抑制率分别为7.1%,57.1%和28.6%。与模型组比较,ECF高剂量组能抑制S180荷瘤小鼠肿瘤的生长(P<0.05),提高胸腺指数(P<0.05),降低血清中M-CSF水平;中剂量组可抑制Lewis肺癌荷瘤小鼠肿瘤的生长(P<0.05),提高胸腺指数(P<0.05),降低血清中M-CSF水平,3个剂量组均能提高血清中TNF-α水平(P<0.05,P<0.01)。结论:ECF对S 180荷瘤小鼠和Lewis肺癌荷瘤小鼠肿瘤的生长具有明显抑制作用,显示较强的体内抗肿瘤活性,其作用机制可能与提高免疫器官指数以及调节血清中TNF-α和M-CSF的表达有关。
OBJECTIVE: To investigate the antitumor activity of Compound Fritillaria ussuriensis (ECF) in vivo and to explore some mechanisms of action. Methods: The mouse models of S180 and Lewis lung carcinoma were prepared. The two solid tumor models were divided into model group, EFC low, medium and high dose (0.05, 0.1, 0.15 g.kg-1) (CTX) positive control group were administered continuously for 8 and 15 days respectively. The effect of ECF on tumor mass, thymus index and spleen index, tumor necrosis factor-α (TNF-α) and tumor necrosis factor-α Effect of macrophage colony stimulating factor (M-CSF) levels. Results: The tumor inhibition rates of S180 tumor-bearing mice were 11.8%, 47.1% and 58.8%, respectively. The tumor inhibition rates of the Lewis lung cancer solid tumor mice were 7.1%, 57.1% And 28.6%. Compared with the model group, ECF high dose group could inhibit tumor growth in S180 tumor-bearing mice (P <0.05), increase thymus index (P <0.05) and decrease serum M-CSF level; (P <0.05), increase thymus index (P <0.05), decrease M-CSF level in serum, and increase the level of TNF-α in serum in three dose groups (P <0.05, P <0.01) ). CONCLUSION: ECF can significantly inhibit the growth of S 180 tumor-bearing mice and Lewis lung carcinoma tumor-bearing mice and show a strong antitumor activity in vivo. The mechanism may be related to the increase of immune organ index and the regulation of serum TNF-α And M-CSF expression.