论文部分内容阅读
目的 探讨肿瘤坏死因子α基因(tumornecrosisfactor -α,TNF -α)启动子区- 30 8和- 2 38位点多态性与中国西南地区汉族人群矽肺易感性的相关性。方法 分别选择96例矽肺患者和116名矽尘接触者为研究对象。采集外周静脉血,应用聚合酶链反应-限制性片段长度多态性分析检测其TNF-α基因多态性。结果 矽肺组中TNF -α- 30 8A、- 2 38A的分布频率显著高于正常矽尘接触组(P<0. 0 1) ;其中I期矽肺患者- 30 8A的分布频率与矽尘接触组差异无统计学意义(P >0 0 1) ,而II期、III期矽肺患者- 30 8A、- 2 38A的分布频率均远高于矽尘接触组(P <0 0 1)。调整混杂因素后,Lo gistic回归分析发现,矽肺组及各亚组和正常矽尘接触组- 30 8A、- 2 38A的分布频率差异均有统计学意义(P <0. 0 1)。结论 TNF -α- 30 8及- 2 38位点基因多态性与中国西南地区汉族人矽肺的发生及其严重程度有关。
Objective To investigate the relationship between polymorphisms of - 30 8 and - 2 38 loci in the promoter region of tumor necrosis factor α (TNF-α) gene and susceptibility to silicosis in Han Chinese population in Southwest China. Methods A total of 96 patients with silicosis and 116 persons with silicosis were selected as study subjects. Peripheral venous blood was collected and the polymorphism of TNF-α gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The distribution frequency of TNF-α-30 8A and -238A in silicosis group was significantly higher than that in normal silica dust exposure group (P <0.01) There was no significant difference between the two groups (P> 0.01). However, the frequency distribution of stage II and stage III silicosis patients - 30 8A and - 2 38A was much higher than that of the silica dust exposure group (P <0.01). After adjustment for confounding factors, Lo gistic regression analysis showed that the distribution frequencies of 30 8A and -2 38A in silicosis group and each subgroup and normal silica exposure group were statistically different (P <0.01). Conclusions The polymorphisms of TNF-α-308 and -238 loci are related to the occurrence and severity of silicosis in Han Chinese in Southwest China.