目的 :通过对胃肠道间质瘤 ( GIST)中原癌基因 c- kit表达的检测 ,探讨 c- kit在GIST中的诊断及临床意义。方法 :用 c- kit、CD34等抗体通过免疫组织化学En Vision法对胃肠道间质瘤进行检测 ,并与平滑肌瘤、神经鞘瘤进行对照研究。结果 :40例 GIST中 c- kit阳性表达率为 97.5 %。CD34阳性表达率为 67.5 %。S- 1 0 0、SMA分别呈 1 7.5 %、1 2 .5 %阳性表达。结蛋白呈阴性表达 ,平滑肌瘤、神经鞘均呈阴性表达。结论 :c- kit作为一种胃肠道间质瘤十分敏感的标记物 ,性能优于 CD34,对 GIST的诊断、鉴别诊断有重要意义。 Objective: To investigate the expression of c-kit in gastrointestinal stromal tumors (GIST) and to evaluate the clinical significance of c-kit in GIST. Methods: Gastrointestinal stromal tumors were detected by immunohistochemical En Vision method using c-kit and CD34 antibodies, and compared with leiomyoma and schwannoma. Results: The positive rate of c-kit in 40 cases of GIST was 97.5%. The positive rate of CD34 expression was 67.5%. S-100 and SMA were 7.5% and 12.5% ​​respectively. Desmin was negative expression, leiomyoma, nerve sheath were negative expression. Conclusion: c-kit, a very sensitive marker of gastrointestinal stromal tumors, is superior to CD34 in diagnosis and differential diagnosis of GIST.