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目的分析牛轮状病毒受体的理化性质及该受体在气管粘膜组织的分布。方法采用ＳＤＳ－聚丙烯酰胺凝胶电泳法（ＳＤＳ－ＰＡＧＥ）、酶消化法、固相分析法对纯化的牛轮状病毒受体进行分析，以免疫组化法检测了新生牛气管粘膜细胞表面牛轮状病毒受体。结果牛轮状病毒受体ＳＤＳ－ＰＡＧＥ分子量为４８０００，该受体对胰蛋白酶及ＰｒｏｎａｓｅＥ蛋白酶敏感，经Ｃｌｏｓｔｒｉｄｉｕｍｐｅｒｆｒｉｎｇｅｎｓ及Ａｒｔｈｒｏｂａｃｔｅｒｕｒｅａｆａｃｉｅｎｓ神经氨酸酶作用后，受体失去结合病毒的生物学活性。免疫组化试验结果为阳性。结论实验结果表明牛轮状病毒受体是结构复杂的糖蛋白，唾液酸残基是受体与牛轮状病毒作用位点的组成部分。新生牛气管粘膜细胞表面有牛轮状病毒受体存在。
Objective To analyze the physicochemical properties of bovine rotavirus receptor and its distribution in tracheal mucosa. Methods Purified bovine rotavirus receptor was analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), enzymatic digestion and solid-phase analysis. Immunohistochemistry was used to detect the cell surface of newborn tracheal mucosa Bovine rotavirus receptor. Results The molecular weight of the bovine rotavirus receptor (SDS-PAGE) was 48,000. The receptor was sensitive to trypsin and PronaseE protease. After the activity of Clostridium perfringens and Arthrobacter urefaciens neuraminidase, the receptor loses the biological activity of binding to the virus. Immunohistochemistry test results were positive. Conclusion The experimental results indicate that the bovine rotavirus receptor is a complex glycoprotein, and sialic acid residues are part of the site of interaction between the receptor and bovine rotavirus. Neonatal tracheal mucosal cell surface bovine rotavirus receptor exists.