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对于小鼠阴栓龄胚第11～17天、19天及生后1天,1～3周新生肾发育过程中组织学和组织化学变化进行了 AcP(Wachstein 改良的 Gomori 方法)、AlP(钙钴法)、ATPase(WachsteinMeisel 中性法)、非特异性酯酶(Pearson 法)的观察,以成年肾做对照。1.肾小体在胚14天,近曲小管、远曲小管、亨利氏襻在胚16天,相继开始分化。2.AlP 活性随肾小体等结构分化完成而减弱,晚期随近曲小管的结构与功能分化渐趋完善而增强。胚16,17天时,肾功能初步建立,此时集合管出现 AcP 和 ATPase 反应强阳性的“附加细胞”,这种细胞在胚19天～初生达最大量,以后逐渐减少,成年肾仅于皮质集合管可见。“附加细胞”出现的意义曾加讨论。3.新生的小鼠肾尚未成熟,AcP、AlP、ATPase 随肾生后的不断发育而变化,其活性强度与分布特点大致都在生后三周左右才接近成年型。4.非特异性酯酶在生后四周才显示活性并伴有明显的性别差异。肾的 AlP 从生后一周已出现性别差异。
The effects of AcP (Wachstein’s modified Gomori method), AlP (calcium), calcium and magnesium on the histological and histochemical changes during neonate kidney development at 11-17 days, 19 days, Cobalt method), ATPase (WachsteinMeisel neutral method), non-specific esterase (Pearson method) observation, with adult kidney as a control. 1. Kidney In the embryo 14 days, near the convoluted tubule, far convoluted tubule, Henrioderma 襻 embryo in the embryo 16 days, one after another began to differentiate. The activity of AlP decreased with the completion of differentiation of renal bodies and other structures, and the structure and function differentiation of proximal tubule became more and more improved. At 16 and 17 days of embryo, renal function was initially established. At this time, there was “extra cell” which was strongly positive for AcP and ATPase in the collecting tube. This cell reached the maximum at 19 days after birth and then decreased gradually. Collection tube visible. The meaning of “additional cells” has been discussed. Neonatal mouse kidney is not yet mature, AcP, AlP, ATPase changes with the continuous development of kidney after birth, its activity intensity and distribution characteristics are almost three weeks after birth close to the adult type. 4. Non-specific esterase showed only four weeks after birth activity and with significant gender differences. Kidney AlP One week after birth, there have been gender differences.