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目的通过观察三维适形调强放疗(Intensity modulated radiation therapy,IMRT)对新疆大鼠继发性骨棘球蚴进行治疗后大鼠T淋巴细胞亚群的影响,探讨IMRT对骨包虫的杀灭机制是否与射线激发宿主对骨包虫有效的免疫反应有关。方法选取股骨继发性棘球蚴病大鼠50只,随机分为IMRT组及空白对照组各25只,以IMRT方式分别对IMRT组25只大鼠左侧股骨棘球蚴病区给予40 Gy剂量的放疗,于放疗1周后所有大鼠活杀取材,取脾制备细胞悬液,用流式细胞术(FCM)检测CD4+/CD8+的值。结果 IMRT作用1周后,流式细胞仪检测结果提示,IMRT组大鼠脾淋巴细胞CD4+/CD8+的值(2.96±1.90)明显高于空白对照组(1.65±1.31),差异具有统计学意义(t=0.544,P<0.05)。结论 IMRT可促进新疆大鼠继发性骨棘球蚴的凋亡,其作用机制可能是与射线激发宿主对骨包虫有效的免疫反应有关。
Objective To observe the effect of three-dimensional Intensity modulated radiation therapy (IMRT) on the T lymphocyte subsets of rats after secondary sporogenetic Echinococcus granulosus in Xinjiang and to investigate the killing effect of IMRT on the hydatid cyst Whether mechanism of action is related to the effective immune response induced by radiation on host. Methods Fifty secondary femoral hydatid disease rats were randomly divided into IMRT group and control group, 25 rats in each group. IMRT group was given 25 rats of IMRT group with 40 Gy After one week of radiotherapy, all the rats were sacrificed and the spleen was taken out to prepare the cell suspension. The flow cytometry (FCM) was used to detect the CD4 + / CD8 +. Results After IMRT for 1 week, the results of flow cytometry indicated that the level of CD4 + / CD8 + of splenic lymphocytes in IMRT group (2.96 ± 1.90) was significantly higher than that in control group (1.65 ± 1.31), the difference was statistically significant ( t = 0.544, P <0.05). Conclusion IMRT can promote the apoptosis of secondary sporogenetic Echinococcus granulosus in Xinjiang. The mechanism may be related to the effective immune response induced by radiation on the host.